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首页> 外文期刊>Bioprocess and Biosystems Engineering >Comparative metabolomic analysis reveals different evolutionary mechanisms for branched-chain amino acids production
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Comparative metabolomic analysis reveals different evolutionary mechanisms for branched-chain amino acids production

机译：比较代谢组学分析揭示了支链氨基酸生产的不同进化机制

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摘要

Evolution is a powerful tool for the breeding of microorganisms, while the connection between the changes of intracellular metabolism and different evolution directions is still unclear, which once clarified, will greatly expand the application of evolutionary engineering. We aim to clarify the correlation between metabolism changes and evolution directions in two Corynebacterium glutamicum strains for l-valine and l-leucine overproducing originated from the same parental strain by repeated random mutagenesis and selection. GC-MS metabolomics was performed to identify and quantify intracellular metabolites of the evolved and wild-type C. glutamicum strains. Time-series comparison of the fermentation processes was performed. The metabolism differences of three strains mainly exist in central carbon metabolism and the stress-resisting modes. C. glutamicum XV developed an overall "pyruvate-saving" mode for l-valine synthesis, and adopted a trehalose accumulating strategy to resist environmental stresses. C. glutamicum CP depended on an enhanced "pyruvate-producing" mode, together with certain "pyruvate-saving" strategies, for efficient l-leucine synthesis, and accumulated proline, my-inositol, and inositol as the stress-resisting measure. These elaborate regulation strategies could be used in future metabolic engineering, making evolution more informative and applicable.
机译：进化是微生物繁殖的有力工具，而细胞内新陈代谢的变化与不同进化方向之间的联系仍然不清楚，一旦阐明，将大大扩展进化工程的应用。我们的目的是通过重复随机诱变和选择，阐明源自同一亲本菌株的两个谷氨酸棒杆菌菌株对L-缬氨酸和L-亮氨酸过量生产的代谢变化与进化方向之间的相关性。进行了GC-MS代谢组学研究，以鉴定和定量进化和野生型谷氨酸棒杆菌菌株的细胞内代谢产物。进行发酵过程的时间序列比较。 3个菌株的代谢差异主要存在于中央碳代谢和抗逆模式。谷氨酸棒杆菌XV开发了用于L-缬氨酸合成的总体“节约丙酮酸”模式，并采用了海藻糖积累策略来抵抗环境胁迫。谷氨酸棒杆菌CP依赖于增强的“丙酮酸生成”模式，以及某些“丙酮酸节省”策略，以实现高效的1-亮氨酸合成，并积累了脯氨酸，肌醇和肌醇作为抗逆措施。这些精心设计的调节策略可用于未来的代谢工程中，从而使进化过程更具参考价值和适用性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2020年第1期|85-95|共11页
  • 作者

    Ma Qian; Mo Xiaolin; Zhang Quanwei; Hou Zhengjie; Tan Miao; Xia Li; Sun Quanwei; Xie Xixian; Chen Ning;

  • 作者单位

    Tianjin Univ Sci Key Lab Ind Fermentation MicroBiol Technology Minist Educ Tianjin People's Republ|Tianjin Univ Sci Tianjin Key Lab Ind MicroBiol Technology Tianjin People's Republ|Tianjin Univ Sci Coll Biotechnology Technology Tianjin People's Republ|Tianjin Univ Sci Local United Engn Lab Metab Control Fermentation Technology Tianjin People's Republ;

    Tianjin Univ Sci Coll Biotechnology Technology Tianjin People's Republ;

    Tianjin Univ Sci Key Lab Ind Fermentation MicroBiol Technology Minist Educ Tianjin People's Republ|Tianjin Univ Sci Tianjin Key Lab Ind MicroBiol Technology Tianjin People's Republ|Tianjin Univ Sci Coll Biotechnology Technology Tianjin People's Republ|Tianjin Univ Sci Local United Engn Lab Metab Control Fermentation Technology Tianjin People's Republ|Tianjin Engn Res Ctr Microbial Metab Fermentation Proc Control Tianjin People's Republ;

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  • 正文语种 eng
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  • 关键词

    Branched-chain amino acid; Corynebacterium glutamcium; Metabolomics; Multivariate statistical analysis;

    机译：支链氨基酸;谷氨酸棒杆菌;代谢组学多元统计分析;

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