...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Design of immobilized biocatalyst and optimal conditions for tyrosol β-galactoside production
【24h】

Design of immobilized biocatalyst and optimal conditions for tyrosol β-galactoside production

机译：固定化生物催化剂的设计和酪蛋白β-半乳糖糖苷生产的最佳条件

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tyrosol beta-galactoside (TG) is a phenylethanoid glycoside with proven neuroprotective properties. This work deals with its biocatalytic production from tyrosol and lactose usingAspergillus oryzae beta-galactosidase in immobilized form. Six commercial carriers were examined to find the optimal biocatalyst. Besides standard biocatalyst performance characteristics, adsorption of the hydrophobic substrate on immobilization carrier matrices was also investigated. The adsorption of tyrosol was significant, but it did not have adverse effects on TG production. On the contrary, TG yield was improved for some biocatalysts. A biocatalyst prepared by covalent binding of beta-galactosidase on an epoxy-activated carrier was used for detailed investigation of the effect of reaction conditions on glycoside production. Temperature had a surprisingly weak effect on the overall process rate. A lactose concentration of 0.83 M was found to be optimal to enhance TG formation. The impact of tyrosol concentration was rather complex. This substrate caused inhibition of all reactions. Its concentration had a strong effect on the hydrolysis of lactose and all products. Higher tyrosol concentrations, 30-40 g/L, were favorable as pseudo-equilibrium concentrations of TG and galactooligosaccharide were reached. Repeated batch results revealed excellent operational stability of the biocatalyst.
机译：酪蛋白β-半乳糖苷（Tg）是具有经过验证的神经保护性能的苯乙醇糖苷。这项工作涉及其从酪术和乳糖的生物催化生产，使用血清酸盐葡萄球菌以固定的形式。检查了六个商业载体以找到最佳的生物催化剂。除标准的生物催化剂性能特征外，还研究了疏水基材对固定载体基质的吸附。酪术的吸附是显着的，但它对TG生产没有不利影响。相反，对于一些生物催化剂，改善了TG产量。通过在环氧活化载体上的β-半乳糖苷酶的共价结合制备的生物催化剂用于详细研究反应条件对糖苷产生的影响。温度对整体过程速率造成令人惊讶的弱点。发现乳糖浓度为0.83μm是最佳的，以增强Tg形成。酪冬醇浓度的影响相当复杂。该基材引起抑制所有反应。它的浓度对乳糖和所有产品的水解产生了很强的影响。随着达到的伪平衡浓度，达到致滴乳菌糖，较高的酪咯醇浓度为30-40克/升浓度。重复的批量结果显示了生物催化剂的优异操作稳定性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号