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Evaluation of inert and organic carriers for Verticillium lecanii spore production in solid-state fermentation

机译:固态发酵中惰性和有机载体对黄萎病菌孢子生产的评价

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摘要

Growth and sporulation of Verticillium lecanii on inert and organic carriers (sugar-cane bagasse, corncob, rice straw, polyurethane foam and activated carbon) in a solid-state fermentation process was studied. Sugar-cane bagasse and polyurethane foam produced 1010 spores g−1 dry carrier whereas corncob, rice straw, and activated carbon yielded, respectively 8 × 109, 4 × 109, and 3 × 108 spores g−1. Chitinase activity of the conidia was in the following order: sugar-cane bagasse (3.3 U mg−1) > wheat bran (3.0 U mg−1) > polyurethane foam (2.7 U mg−1). There was no significant difference (2.5–2.7 U mg−1) in the proteinase activity among the conidia from the three cultures. Scanning electron microscopy shows that aerial mycelium freely penetrated into the internal area of polyurethane foam. Sugar-cane bagasse provided enough area for vegetative hyphae to attach. Of the carriers analyzed, polyurethane foams and sugar-cane bagasse were the best carriers for V. lecanii growth and spore production.
机译:研究了黄萎病菌在固态发酵过程中在惰性和有机载体(甘蔗渣,玉米芯,稻草,聚氨酯泡沫和活性炭)上的生长和孢子形成。甘蔗渣和聚氨酯泡沫产生10 10 孢子g -1 干载体,而玉米芯,稻草和活性炭分别产生8×10 9 < / sup>,4×10 9 和3×10 8 孢子g -1 。分生孢子的几丁质酶活性按以下顺序排列:甘蔗渣(3.3 U mg -1 )>麦麸(3.0 U mg -1 )>聚氨酯泡沫( 2.7 U mg -1 )。三种培养物的分生孢子之间的蛋白酶活性没有显着差异(2.5–2.7 U mg -1 )。扫描电子显微镜显示,气生菌丝体自由渗透到聚氨酯泡沫的内部区域。甘蔗渣提供了足够的营养菌丝附着区域。在分析的载体中,聚氨酯泡沫和甘蔗渣是V. lecanii生长和孢子产生的最佳载体。

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