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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Effect of in vivo passage on spore germination and virulence of Verticillium lecanii
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Effect of in vivo passage on spore germination and virulence of Verticillium lecanii

机译：体内通道对孢子萌发和血管毒力的影响

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The pathogen was passed through Brevicoryne brassicae, for 10 generations and after each passage, virulence of re-isolated fungus was tested on the aphid. Simultaneously, the fungus was re-cultured from the stock culture on PDA medium. During the first five passages of V. lecanii through B. brassicae the germination of fungal spores showed no significant difference. At 6~(th), 7~(th) and 9~(th) passages spore germination slightly increased. Maximum spore germination was observed at 9th and 10th passages which was significantly higher than other generations. Germination of spores without in vivo passage showed significant decline at 2~(nd) generation. From 4~(th) generation to 7~(th) generation, spore germinations were on par and significant decrease again was observed from 8th generation. Aphid mortality after 2~(nd) and 9~(th) passages significantly increased. Mortality caused by the fungus without passing through the aphid significantly decreased in 4~(th), 5~(th), 7~(th), 8~(th) and 9~(th) generations of sub-culturing.
机译：将病原体通过Brevicioryne Brassicae，10代和每篇通道后，在蚜虫上测试重新分离的真菌的毒力。同时，真菌从PDA培养基上重新培养。在V.Lecanii的前五个通道通过B. Brassicae进行真菌孢子的萌发显示没有显着差异。在6〜（th），7〜（th）和9〜（th）通道孢子萌发略有增加。在第9和第10个通道中观察到最大孢子萌发，其明显高于其他世代。没有体内通道的孢子萌发显示出2〜（ND）产生的显着下降。从4〜（th）至7〜（th）产生，从第8代观察到孢子萌发是对靶标并再次显着降低。 2〜（nd）和9〜（Th）通道后的蚜虫死亡率显着增加。在没有通过蚜虫的真菌引起的死亡率在4〜（th），5〜（Th），7〜（Th），8〜（Th）和9〜（Th）代和9〜（Th）几代副作用中显着降低。

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