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Oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus

机译：寡核苷酸芯片，实时PCR和测序技术用于乙型肝炎病毒的基因分型

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摘要

AIM: To compare the oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus in Chinese patients with chronic hepatitis B. METHODS: Mixture of samples with different genotypes and clinical serum samples from 126 chronic hepatitis B patients was tested for hepatitis B virus genotypes by oligonucleotide chip, real-time PCR and sequencing of PCR products, respectively. Clinical performances, time required and costs of the three assays were evaluated. RESULTS: Oligonucleotide chips and real-time PCR detected 1% and 0.1% genotypes, respectively, in mixed samples. Of the 126 clinical samples from patients with chronic hepatitis B, genotype B was detected in 41 (33%), 41 (33%) and 45 (36%) samples, and genotype C in 76 (60%), 76 (60%) and 81 (64%) samples, by oligonucleotide chip, real-time PCR and sequencing, respectively. Oligonucleotide chip and real-time PCR detected mixed genotypes B and C in 9 samples. Realtime PCR was the rapidest and cheapest among the three assays. CONCLUSION: Oligonucleotide chip and real-time PCR are able to detect mixed genotypes, while sequencing only detects the dominant genotype in clinical samples.
机译：目的：为了比较寡核苷酸芯片，实时PCR和测序技术对中国慢性乙型肝炎患者乙型肝炎病毒的基因分型。方法：测试了126名慢性乙型肝炎患者不同基因型的样本和临床血清样本的混合物分别通过寡核苷酸芯片，实时PCR和PCR产物测序确定B病毒的基因型。评估了三种测定的临床表现，所需时间和成本。结果：混合样本中的寡核苷酸芯片和实时PCR分别检测到1％和0.1％的基因型。在来自慢性乙型肝炎患者的126个临床样本中，在41个（33％），41个（33％）和45个（36％）样本中检测到B基因型，在76个（60％），76个（60％）中检测到C基因型）和81（64％）个样品，分别通过寡核苷酸芯片，实时PCR和测序。寡核苷酸芯片和实时PCR检测了9个样品中的混合基因型B和C。在这三种测定中，实时PCR最快，最便宜。结论：寡核苷酸芯片和实时荧光定量PCR能够检测混合基因型，而测序只能检测临床样品中的显性基因型。

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