• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >'Defective' mutations of hepatitis D viruses in chronic hepatitis D patients.
【24h】

'Defective' mutations of hepatitis D viruses in chronic hepatitis D patients.

机译：慢性D型肝炎患者中D型肝炎病毒的“缺陷”突变。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To verify whether "defective" mutations existed in hepatitis D virus (HDV). METHODS: Hepatitis delta antigen (HDAg)-coding sequences were amplified using Pfu DNA polymerases with proof-reading activities from sera of five patients with chronic hepatitis D. Multiple colonies were sequenced for each patient. Pfu analyzed a total of 270 HDV clones. Three representative defective HDV clones were constructed in expression plasmids and transfected into a human hepatoma cell line. Cellular proteins were extracted and analyzed by Western blot. RESULTS: Four of five cases (80%) showed defective HDV genomes in their sera. The percentage of defective genomes was 3.7% (10/270). The majority (90%) of the defective mutations were insertions or deletions that resulted in frameshift and abnormal stop translation of the HDAg. The predicted mutated HDAg ranged from 45 amino acids to >214 amino acids in length. Various domains of HDAg associated with viral replication or packaging were affected in different HDV isolates. Western blot analysis showed defected HDAg in predicted positions. CONCLUSION: "Defective" viruses do exist in chronic HDV infected patients, but represented as minor strains. The clinical significance of the "defected" HDV needs further study to evaluate.
机译：目的：验证丁型肝炎病毒（HDV）中是否存在“缺陷”突变。方法：使用具有5个慢性D型肝炎患者血清检测活性的Pfu DNA聚合酶扩增肝炎三角洲抗原（HDAg）编码序列。对每个患者进行多个菌落测序。 Pfu共分析了270个HDV克隆。在表达质粒中构建了三个代表性的缺陷HDV克隆，并转染到人肝癌细胞系中。提取细胞蛋白并通过蛋白质印迹分析。结果：五分之四的患者（80％）血清中存在HDV基因组缺陷。缺陷基因组的百分比为3.7％（10/270）。大多数缺陷突变（90％）是导致HDAg移码和异常终止翻译的插入或缺失。预测的突变的HDAg的长度范围为45个氨基酸至> 214个氨基酸。在不同的HDV分离物中，与病毒复制或包装相关的HDAg的各个域均受到影响。蛋白质印迹分析显示，HDAg在预期位置存在缺陷。结论：“缺陷型”病毒确实存在于慢性HDV感染患者中，但代表较小毒株。 “变形的” HDV的临床意义需要进一步研究以评估。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号