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首页> 外文期刊>World Journal of Gastroenterology >Kinetics of phytohemaglutinin-induced IFN-γ and TNF-α expression in peripheral blood mononuclear cells from patients with chronic hepatitis B after liver transplantation
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Kinetics of phytohemaglutinin-induced IFN-γ and TNF-α expression in peripheral blood mononuclear cells from patients with chronic hepatitis B after liver transplantation

机译：肝移植后植物血凝素诱导的慢性乙型肝炎患者外周血单个核细胞中IFN-γ和TNF-α表达的动力学

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AIM: To study the association between host immunity and hepatitis B virus (HBV) recurrence after liver transplantation. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from 40 patients with hepatitis B and underwent orthotopic liver transplantation (OLT) before and 2,4,8 wk after surgery. After being cultured in vitro for 72 h, the levels of INF-γ and TNF-α in culture supernatants were detected with ELISA. At the same time, the quantities of HBV DNA in serum and PBMCs were measured by real time PCR. RESULTS: The levels of INF-γ and TNF-α in PBMC culture supernatants decreased before and 2, 4 wk after surgery in turns (INF-γ 155.52±72.32 ng/L vs 14.76±9.88 ng/L vs 13.22±10.35 ng/L, F= 6.946, P= 0.027< 0.05; TNF-α 80.839±46.75 ng/L vs 18.59±17.29 ng/L vs 9.758±7.96 ng/L, F = 22.61, P= 0.0001 < 0.05). The levels of INF-γ and TNF-α were higher in groups with phytohemagglutinin (PHA) than in those without PHA before surgery. However, the difference disappeared following OLT. Furthermore, INF-γ and TNF-α could not be detected in most patients at wk 4 and none at wk 8 after OLT. The HBV detection rate and virus load in PBMC before and 2,4 wk after surgery were fluctuated (HBV detected rate: 51.4%, 13.3%, 50% respectively; HBV DNA: 3.55±0.674 log(10) copies/mL vs 3.00±0.329 log(10) copies/mL vs 4.608±1.344 log(10) copies/mL, F= 7.582, P= 0.002 < 0.05). HBV DNA in serum was 4.48±1.463 log(10) copies/mL before surgery and < 10~3 copies/mL after OLT except for one with 5.72x10~6 copies/mL 4 wk after OLT who was diagnosed as HBV recurrence. The levels of INF-γ and TNF-α were lower in patients with a high HBV load than in those with a low HBV load (HBV DNA detected/undetected in PBMCs: IFN-γ 138.08±72.44 ng/L vs164.24±72.07 ng/L, t= 1.065, P= 0.297 > 0.05, TNF-α 80.75±47.30 ng/L vs 74.10±49.70 ng/L, t= 0.407, P= 0.686 > 0.05; HBV DNA positiveegative: IFN-γ 136.77±70.04 ng/L vs 175.27±71.50 ng/L, t= 1.702, P= 0.097 > 0.05; TNF-α 75.37±43.02 ng/L vs 81.53±52.46 ng/L, t= 0.402, P= 0.690 > 0.05). CONCLUSION: The yielding of INF-γ and TNF-α from PBMCs is inhibited significantly by immunosuppressive agents following OLT with HBV load increased, indicating that the impaired immunity of host is associated with HBV recurrence after OLT.
机译：目的：研究宿主免疫与肝移植术后乙型肝炎病毒（HBV）复发之间的关系。方法：从40例乙型肝炎患者中分离出外周血单个核细胞（PBMC），分别于术前和术后2、4、8周进行原位肝移植（OLT）。体外培养72小时后，用ELISA检测培养上清液中的INF-γ和TNF-α水平。同时，通过实时PCR测量血清和PBMC中HBV DNA的量。结果：PBMC培养上清液中INF-γ和TNF-α的水平在术前和术后2 wk依次降低（INF-γ155.52±72.32 ng / L对14.76±9.88 ng / L对13.22±10.35 ng / L L，F ＝ 6.946，P ＝ 0.027 ＜0.05；TNF-α80.839±46.75ng / L对18.59±17.29ng / L对9.758±7.96ng / L，F ＝ 22.61，P ＝ 0.0001 ＜0.05）。有植物血凝素（PHA）的组的INF-γ和TNF-α水平高于没有术前血凝素的组。但是，OLT之后差异消失了。此外，大多数患者在OLT后第4周和第8周都未检测到INF-γ和TNF-α。术前和术后2,4 wk的PBMC中HBV检测率和病毒载量波动（HBV检测率分别为51.4％，13.3％，50％; HBV DNA：3.55±0.674 log（10）个拷贝/ mL与3.00± 0.329 log（10）拷贝/ mL与4.608±1.344 log（10）拷贝/ mL，F = 7.582，P = 0.002 <0.05）。术前血清HBV DNA为4.48±1.463 log（10）拷贝/ mL，OLT后为<10〜3拷贝/ mL，但OLT后4周有5.72x10〜6拷贝/ mL被诊断为HBV复发。 HBV高负荷患者的INF-γ和TNF-α水平低于HBV低负荷患者（PBMC中检测到/未检测到HBV DNA：IFN-γ138.08±72.44 ng / L vs164.24±72.07 ng / L，t = 1.065，P = 0.297> 0.05，TNF-α80.75±47.30 ng / L vs 74.10±49.70 ng / L，t = 0.407，P = 0.686> 0.05; HBV DNA阳性/阴性：IFN-γ 136.77±70.04 ng / L与175.27±71.50 ng / L，t = 1.702，P = 0.097> 0.05;TNF-α75.37±43.02 ng / L与81.53±52.46 ng / L，t = 0.402，P = 0.690> 0.05 ）。结论OLT HBV负荷增加后，免疫抑制剂可明显抑制PBMCs的INF-γ和TNF-α的产生，表明宿主免疫力降低与OLT后HBV复发有关。

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