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首页> 外文期刊>World Journal of Gastroenterology >Cysteamine increases expression and activity of H+-K+-ATPase of gastric mucosal cells in weaning piglets.
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Cysteamine increases expression and activity of H+-K+-ATPase of gastric mucosal cells in weaning piglets.

机译：半胱胺能提高断奶仔猪胃粘膜细胞H + -K + -ATP酶的表达和活性。

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AIM: To determine the in vivo and in vivo effects of cysteamine (CS) on expression and activity of H(+)-K(+)-ATPase of gastric mucosal cells in weaning piglets. METHODS: Eighteen litters of newborn Xinhuai piglets were employed in the in vivo experiment and allocated to control and treatment groups. From 12 d of age (D12), piglets in control group were fed basal diet, while the treatment group received basal diet supplemented with 120 mg/kg CS. Piglets were weaned on D35 in both groups. Six piglets from each group (n = 6) were slaughtered on D28 (one week before weaning), D35 (weaning), D36.5, D38, D42, and D45 (36 h, 72 h, one week and 10 d after weaning), respectively. Semi-quantitative RT-PCR was performed to determine the levels of H(+)-K(+)-ATPase mRNA in gastric mucosa. H(+)-K(+)-ATPase activity in gastric mucosa homogenate was also determined. Gastric mucosal epithelial cells from piglets through primary cultures were used to further elucidate the effect of CS on expression and activity of H(+)-K(+)-ATPase in vivo. Cells were treated for 20 h with 0.001, 0.01, and 0.1 mg/mL of CS (n = 4), respectively. The mRNA expression of H(+)-K(+)-ATPase and somatostatin (SS) as well as the H(+)-K(+)-ATPase activity were determined. RESULTS: in vivo, both mRNA expression and activity of H(+)-K(+)-ATPase in gastric mucosa of control group exhibited a trend to increase from D28 to D45, reaching a peak on D45, but did not show significant age differences. Furthermore, neither the mRNA expression nor the activity of H(+)-K(+)-ATPase was affected significantly by weaning. CS increased the mRNA expression of H(+)-K(+)-ATPase by 73%, 53%, 30% and 39% on D28 (P = 0.014), D35 (P = 0.017), D42 (P = 0.013) and D45 (P = 0.046), respectively. In accordance with the mRNA expression, H(+)-K(+)-ATPase activities were significantly higher in treatment group than in control group on D35 (P = 0.043) and D45 (P = 0.040). In vivo, CS exhibited a dose-dependent effect on mRNA expression and activity of H+-K+-ATPase. Both H(+)-K(+)-ATPase mRNA expression and activity in gastric mucosal epithelial cells were significantly elevated after 20 h of exposure to the moderate (H(+)-K(+)-ATPase expression: P=0.03; H(+)-K(+)-ATPase activity: P = 0.014) and high concentrations (H(+)-K(+)-ATPase expression: P=0.017; H(+)-K(+)-ATPase activity: P = 0.022) of CS. Significant increases in SS mRNA expression were observed to accompany the elevation of H(+)-K(+)-ATPase expression and activity induced by the moderate (P = 0.024) and high concentrations (P = 0.022) of CS. Low concentration of CS exerted no effects either on expression and activity of H(+)-K(+)-ATPase or on SS mRNA expression in cultured gastric mucosal epithelial cells. CONCLUSION: No significant changes are observed in mRNA expression and activity of H(+)-K(+)-ATPase in gastric mucosa of piglets around weaning from D28 to D45. CS increases expression and activity of gastric H(+)-K(+)-ATPase in vivo and in vivo. SS is involved in mediating the effect of CS on gastric H(+)-K(+)-ATPase expression and activity in weaning piglets.
机译：目的：确定半胱胺（CS）对断奶仔猪胃粘膜细胞H（+）-K（+）-ATPase表达和活性的体内和体内影响。方法：将18只新生仔猪仔猪用于体内实验，并分为对照组和治疗组。对照组（D12）从12日龄开始给仔猪饲喂基础饲料，治疗组接受基础饲料补充120 mg / kg CS。两组均在D35断奶。每组六只仔猪（n = 6）在断奶后D28（断奶前一周），D35（断奶），D36.5，D38，D42和D45屠宰（断奶后36小时，72小时，1周和10天） ）， 分别。进行半定量RT-PCR以确定胃粘膜中H（+）-K（+）-ATPase mRNA的水平。还确定了胃粘膜匀浆中的H（+）-K（+）-ATPase活性。仔猪通过原代培养的胃粘膜上皮细胞用于进一步阐明CS对体内H（+）-K（+）-ATPase的表达和活性的影响。将细胞分别用0.001、0.01和0.1 mg / mL的CS（n = 4）处理20 h。测定了H（+）-K（+）-ATPase和生长抑素（SS）的mRNA表达以及H（+）-K（+）-ATPase的活性。结果：在体内，对照组胃黏膜中H（+）-K（+）-ATPase的mRNA表达和活性均呈现出从D28增加到D45的趋势，在D45达到峰值，但年龄不显着。差异。此外，断奶不会显着影响mRNA的表达或H（+）-K（+）-ATPase的活性。 CS使D28（P = 0.014），D35（P = 0.017），D42（P = 0.013）上H（+）-K（+）-ATPase的mRNA表达增加73％，53％，30％和39％和D45（P = 0.046）。根据mRNA的表达，在D35组（P = 0.043）和D45（P = 0.040），治疗组的H（+）-K（+）-ATPase活性明显高于对照组。在体内，CS对H + -K + -ATPase的mRNA表达和活性表现出剂量依赖性。暴露于中度（H（+）-K（+）-ATPase表达20小时后，胃粘膜上皮细胞中的H（+）-K（+）-ATPase mRNA表达和活性均显着升高：P = 0.03； H（+）-K（+）-ATPase活性：P = 0.014）和高浓度（H（+）-K（+）-ATPase表达：P = 0.017; H（+）-K（+）-ATPase活性：P ＝ 0.022）。观察到SS mRNA表达的显着增加伴随着中度（P = 0.024）和高浓度（P = 0.022）的CS诱导的H（+）-K（+）-ATPase表达和活性的升高。低浓度的CS既不影响H（+）-K（+）-ATPase的表达和活性，也不影响培养的胃粘膜上皮细胞的SS mRNA表达。结论：断奶仔猪从D28到D45前后，胃黏膜中H（+）-K（+）-ATPase的mRNA表达和活性均未见明显变化。 CS增加体内和体内胃H（+）-K（+）-ATPase的表达和活性。 SS参与调解CS对断奶仔猪胃H（+）-K（+）-ATPase表达和活性的影响。

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