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首页> 外文期刊>World Journal of Gastroenterology >Effects of different ingredients of zedoary on gene expression of HSC-T6 cells.
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Effects of different ingredients of zedoary on gene expression of HSC-T6 cells.

机译：虫不同成分对HSC-T6细胞基因表达的影响。

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AIM: To investigate the effects of four different ingredients of zedoary (Curcuma aromatica oil, Curcumol, beta-elemence, and Curcumin) on the gene expressions of hepatic stellate cells (HSCs), and to explore the molecular mechanism of zedoary against hepatic fibrosis at gene network level.METHODS: We detected the mRNA sequences of 50 liver fibrosis-related genes in GenBank and designed oligonucleotide probes. We synthesized oligonucleotides with PE8909 DNA synthesizing instrument, and carried out oligonucleotide microarray with OGR-04 dropping instrument and aldehyded glass chip. Cultured HSC-T6 cells were treated with different concentrations of Colchicine, Curcuma aromatica oil, Curcumol, beta-elemence, and Curcumin. According to the experiment of cell toxicity, we took the appropriate concentrations of medicines that resulted in over 50% of cell survival as experiment concentrations. We collected the cells at 1, 6, 12, and 24 h, and extracted total RNA with TRIzol reagent, then labeled cDNAs with Cy3-dUTP and Cy5-dUTP. These labeled cDNAs were hybridized to an oligonucleotide microarray which was washed several times and scanned by scanner GenePix 4000B. Different gene expressions of HSC-T6 cells were analyzed by ImaGene 4.2 software.RESULTS: After HSC-T6 cells were cultured in a medium containing 6.25 microg/mL Colchicine for 12 h, expression of TIMP-1 decreased 2.2-folds. After HSC-T6 cells were cultured in a medium containing 78.125 microg/mL of Curcuma aromatica oil for 24 h, the expression of TIMP-2 and IL-6 decreased 2.3- and 2.2-folds, respectively. Moreover, after HSC-T6 cells were cultured in a medium containing 1.5625 microg/mL of Curcumol for 12 h, the expression of TGFbeta1 and P450a decreased 2.3- and 2.1-folds, respectively.CONCLUSION: Our results may show the possible molecular mechanism of Curcuma aromatica oil and Curcumol against hepatic fibrosis.
机译：目的：探讨姜黄素的四种不同成分（姜黄，姜黄素，β-香精和姜黄素）对肝星状细胞（HSCs）基因表达的影响，并探讨姜黄素抗肝纤维化的分子机制。方法：我们在GenBank中检测了50种肝纤维化相关基因的mRNA序列，并设计了寡核苷酸探针。我们用PE8909 DNA合成仪合成了寡核苷酸，并用OGR-04滴液仪和醛玻璃芯片进行了寡核苷酸微阵列分析。将培养的HSC-T6细胞用不同浓度的秋水仙碱，姜黄油，姜黄素，β-香精和姜黄素处理。根据细胞毒性实验，我们将导致细胞存活率超过50％的适当药物浓度作为实验浓度。我们在1、6、12和24小时收集细胞，并用TRIzol试剂提取总RNA，然后用Cy3-dUTP和Cy5-dUTP标记cDNA。将这些标记的cDNA与寡核苷酸微阵列杂交，将其洗涤几次并用扫描仪GenePix 4000B扫描。结果：在含6.25微克/毫升秋水仙碱的培养基中培养12小时后，TIMP-1的表达下降了2.2倍。通过ImaGene 4.2软件分析了HSC-T6细胞的不同基因表达。将HSC-T6细胞在含有78.125微克/毫升姜黄油的培养基中培养24小时后，TIMP-2和IL-6的表达分别下降2.3倍和2.2倍。此外，在含有1.5625 microg / mL姜黄素的培养基中培养HSC-T6细胞12 h后，TGFbeta1和P450a的表达分别降低了2.3倍和2.1倍。姜黄芳香油和姜黄醇可抗肝纤维化。

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