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首页> 外文期刊>World Journal of Gastroenterology >Anti-neoplastic efficacy of Haimiding on gastric carcinoma and its mechanisms
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Anti-neoplastic efficacy of Haimiding on gastric carcinoma and its mechanisms

机译：海密定对胃癌的抗肿瘤作用及其机制

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AIM: To study the anti-neoplastic effect of Haimiding and its mechanisms of action. METHODS: Experiments using MTT and colony formation were carried out to study the in vitro anti-neoplastic action of Haimiding, its in vivo anti-neoplastic action was studied by observing its effect on the weight of tumors in FC mice and S_(180), H_(22) tumor bearing mice, as well as their life spans. The effect of Haimiding on cell apoptosis and different stages of cell cycles in human gastric carcinoma cells were studied by flow cytometry. Its effect on [Ca~(2+)]_i of human gastric carcinoma cells and the source of Ca~(2+) during the change of [Ca~(2+)]_i were observed by confocal laser scanning technique. RESULTS: Haimiding showed a definite cytotoxicity to 8 human tumor cell lines, which was most prominent against BGC-823, E_(ca-109) and HCT-8 tumor cells. It also exhibited an obvious inhibition on colony formation of the above tumor cell lines, which was most prominent in E_(ca-109) tumor cells. It showed obvious inhibition on the growth of tumor in FC mice and S_(108) bearing mice as well as prolonged the life span of H_(22) bearing mice. It was able to induce apoptosis and elevate intracellular [Ca~(2+)]_i concentration of tumor cells. The source of Ca~(2+) came from both extracellular Ca~(2+) influx and intracellular Ca~(2+) release. CONCLUSION: Haimiding is composed of aTCM preparation and 5-flurouracil. Its anti-neoplastic potency is highly enhanced by synergism as compared with either one of its components. Its mechanisms of anti-neoplastic action can be attributed to its action to initiate apoptosis of tumor cells by opening the membrane calcium channel and inducing intracellular Ca~(2+) release to elevate [Ca~(2+)]_i of the tumor cells.
机译：目的：研究海米定的抗肿瘤作用及其作用机理。方法：采用MTT和菌落形成实验研究海米定的体外抗肿瘤作用，观察其对FC小鼠和S_（180）小鼠肿瘤重量的影响，研究其体内抗肿瘤作用。 H_（22）荷瘤小鼠及其寿命。流式细胞术研究了海米定对人胃癌细胞凋亡和细胞周期不同阶段的影响。通过共聚焦激光扫描技术观察了其对人胃癌细胞[Ca〜（2 +）] _ i的影响以及Ca〜（2+）来源的变化。结果：Haimiding对8种人类肿瘤细胞系表现出一定的细胞毒性，其中最显着的抗BGC-823，E_（ca-109）和HCT-8肿瘤细胞。它还对上述肿瘤细胞系的集落形成表现出明显的抑制作用，这在E_（ca-109）肿瘤细胞中最为明显。它对FC小鼠和荷S_（108）小鼠的肿瘤生长具有明显的抑制作用，并延长了荷H_（22）小鼠的寿命。它能够诱导细胞凋亡并提高肿瘤细胞的细胞内[Ca〜（2 +）] _ i浓度。 Ca〜（2+）的来源既来自细胞外Ca〜（2+）的流入，又来自细胞内Ca〜（2+）的释放。结论：海米定由aTCM制剂和5-氟尿嘧啶组成。与任一成分相比，其协同作用可大大增强其抗肿瘤潜能。其抗肿瘤作用的机制可归因于其通过打开膜钙通道并诱导细胞内Ca〜（2+）释放以升高肿瘤细胞[Ca〜（2 +）] _ i来启动肿瘤细胞凋亡的作用。 。

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