• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Helicobacter species sequences in liver samples from patients with and without hepatocellular carcinoma
【24h】

Helicobacter species sequences in liver samples from patients with and without hepatocellular carcinoma

机译：有和没有肝细胞癌患者肝样本中的幽门螺杆菌物种序列

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: Only a minority of patients carrying a defined viral aetiologic agent develop cirrhosis and ultimately hepatocellular carcinoma (HCC), the mechanism underlying the worsening is still undefined. Experimental infection by Helicobacter hepaticus in mice causes chronic hepatitis and HCC and recently, more Helicobacterspecies (Helicobacter spp.) have been detected in the liver of patients suffering from cholestatic diseases and HCC arising from non-cirrhotic liver. We investigated whether Helicobacterspp. sequences could be detected in the liver of patients with cirrhosis and HCC compared to subjects with metastasis to liver from colon cancer. METHODS: Twenty-three liver samples from patients operated upon for HCC superimposed on hepatitis C virus (HCV)-related cirrhosis and 6 from patients with resected metastases from colorectal cancer, were tested by polymerase chain reaction for presence of genomic 16S rRNA of Helicobactergenus using specific primers. DNA sequencing and cag A gene analysis were also performed. RESULTS: Genomic sequences of Helicobacter spp. were found in 17 of 20 (85%) liver samples from patients with HCC and in 2 of 6 samples from patients with liver metastasis. In three samples of the first group the result was uncertain. H pylori was revealed in 16 out of 17 positive samples and Helicobacter pullorum in the other. CONCLUSION: Helicobacter spp., carcinogenic in mice, were found at a higher frequency in the liver of patients with HCV-related cirrhosis and HCC than those in patients without primary liver disease.
机译：目的：只有少数携带确定的病毒病原体的患者会发展为肝硬化，最终发展为肝细胞癌（HCC），而导致这种情况恶化的机制仍不确定。小鼠肝杆菌感染的实验性感染可导致慢性肝炎和HCC，最近，在胆汁淤积性疾病和非肝硬化性肝癌引起的HCC患者的肝中发现了更多的幽门螺杆菌（Helicobacter spp。）。我们调查了Helicobacterspp。与从结肠癌转移至肝的受试者相比，可以在肝硬化和HCC患者的肝脏中检测到这些序列。方法：通过聚合酶链反应，使用聚合酶链反应检测了23例接受丙型肝炎病毒（HCV）相关性肝硬化的HCC手术患者和6例结直肠癌切除转移患者的肝样本，并使用幽门螺杆菌检测了16S rRNA基因特异性引物。还进行了DNA测序和cag A基因分析。结果：Helicobacter spp的基因组序列。在HCC患者的20份肝样本中有17份（占85％）和肝转移患者的6份样本中有2份被发现。在第一组的三个样本中，结果不确定。幽门螺杆菌在17个阳性样品中的16个中被揭示，而在另一个样品中则显示了鸡白痢。结论：与非原发性肝病患者相比，丙型肝炎病毒相关性肝硬化和肝癌患者在肝脏中发现致癌的幽门螺杆菌的频率更高。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号