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首页> 外文期刊>World Journal of Gastroenterology >Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells
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Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

机译：丙型肝炎病毒包膜蛋白2的表达诱导培养的哺乳动物细胞凋亡

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摘要

AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by ~3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells.
机译：目的：探讨丙型肝炎病毒（HCV）包膜蛋白2（E2）在诱导细胞凋亡中的作用。方法：羧基端截短的E2（E2-661）在几种培养的哺乳动物细胞系中瞬时表达或在中国仓鼠卵巢（CHO）细胞系中稳定表达。通过〜3H胸腺嘧啶核苷摄取评估细胞增殖。通过Hoechst 33258染色，流式细胞术和DNA片段化分析检查细胞凋亡。结果：在表达E2-661的细胞中很容易观察到增殖减少。这些细胞表现出凋亡的典型特征，包括细胞收缩，染色质浓缩和二倍体基因组DNA含量。在稳定表达的E2-661细胞系中观察到了类似的凋亡细胞死亡。结论：HCV E2可诱导培养的哺乳动物细胞凋亡。

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