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首页> 外文期刊>World Journal of Gastroenterology >Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus
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Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus

机译：追踪含有甲型和非甲型乙型肝炎病毒的接种物对查马克狒狒的感染

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摘要

AIM: To determine whether one genotype (A or non-A genotypes of HBV) predominated over the other during the course of HBV infection. METHODS: Four baboons were inoculated with HBV. DNA was extracted from serum obtained at monthly intervals post-inoculation for 52 weeks and HBV DNA was amplified using primers specific for the core region containing an insert characteristic of genotype A (nt 2 354-2 359, numbering from the EcoRI site). The amplicons were cloned into PCR-Script~(TM) and a minimum of 15 clones per time point were sequenced in both directions. RESULTS: Both genotypes persisted for the entire follow-up period of 52 weeks. Genotype non-A predominated in two baboons and genotype A in one baboon. Neither genotype predominated in the fourth baboon, as shown at a 5 % level of testing. CONCLUSION: No conclusions concerning the dominance of either genotype or the natural progression or replication rates of HBV could be drawn because the pattern of the genotypes found may have been caused by sampling fluctuations at the time of DNA extraction and cloning as a result of the very low viral loads in the baboon sera.
机译：目的：确定在HBV感染过程中，一种基因型（HBV的A型或非A基因型）是否优先于另一种。方法：将四个狒狒接种乙肝病毒。从接种后每月间隔52周的血清中提取DNA，并使用对核心区域具有特异性的引物扩增HBV DNA，该核心区域包含基因型A的插入特征（nt 2 354-2 359，从EcoRI位点编号）。将扩增子克隆到PCR-Script TM中，并且每个时间点在两个方向上最少测序15个克隆。结果：这两种基因型在整个52周的随访期内均持续存在。非狒狒基因型在两个狒狒中占主导地位，而基因型A在一个狒狒中。测试水平为5％时，第四个狒狒均未占主导地位。结论：关于基因型的优势或HBV的自然进展或复制率的结论尚无定论，因为发现的基因型模式可能是由于DNA提取和克隆时的样本波动导致的，狒狒血清中病毒载量低。

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