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首页> 外文期刊>World Journal of Gastroenterology >Effects of hepatitis B virus infection on human sperm chromosomes
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Effects of hepatitis B virus infection on human sperm chromosomes

机译：乙肝病毒感染对人类精子染色体的影响

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AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients. METHODS: Sperm chromosomes of 14 tested subjects (5 healthy controls, 9 patients with HBV infection, including 1 with acute hepatitis B, 2 with chronic active hepatitis B, 4 with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes. RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8 %, 33/223) was significantly higher than that in the control group (4.3 %, 5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4 signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random. CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.
机译：目的：评估男性乙型肝炎患者精子染色体畸变水平，并直接检测乙型肝炎患者精子染色体中是否存在HBV DNA整合。方法：对14名受检者的精子染色体（5名健康对照者，9名HBV感染患者，包括1例急性乙型肝炎，2例慢性活动性乙型肝炎，4例慢性持续性乙型肝炎，2例无临床症状的慢性HBsAg携带者）进行了研究。用无区带金仓鼠卵和人类精子之间的种间体外受精技术制备，并比较HBV感染受试者和对照组的精子畸变频率。用生物素标记的全长HBV DNA探针对精子染色体扩散进行荧光原位杂交（FISH），以检测精子染色体中的特定HBV DNA序列。结果：HBV感染组精子染色体畸变的总频率（14.8％，33/223）明显高于对照组（4.3％，5/116）。此外，HBV感染患者的精子染色体通常表现出粘性，结块，染色失败等，这会影响精子染色体的分析。在一名慢性持续性肝炎患者的精子染色体中发现了HBV DNA的特异性荧光信号点。在9个（9/42）包含荧光信号斑点的精子染色体补体中，一个呈现5个明显的FISH斑点，其他呈现2至4个信号。信号点之间的荧光强度存在显着差异。染色体之间信号位点的分布是随机的。结论：HBV感染可对精子染色体产生诱变作用。病毒DNA整合到多部位且非特异性的精子染色体中会进一步增加精子染色体的不稳定性。这项研究表明，HBV感染可通过改变遗传成分和/或诱导染色体畸变以及通过种系垂直传播HBV到下一代而产生广泛的遗传效应。

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