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首页> 外文期刊>World Journal of Gastroenterology >Action of progesterone on contractile activity of isolated gastric strips in rats
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Action of progesterone on contractile activity of isolated gastric strips in rats

机译：孕酮对大鼠离体胃条收缩活性的作用

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AIM: To study the effect of progesterone on contractile activity of isolated gastric strips in rats. METHODS: Wistar rats were sacrificed to remove whole stomach. Then, the stomach was opened and the mucosal layer was removed. Parellel to either the circular or the longitudial fibers, muscle strips were cut from fundus, body, antrum and pylorus. Each muscle strip was suspended in a tissue chamber containing 5 mL Krebs solution. Then the motility of gastric strips in tissue chambers was simultaneously recorded. The preparations were subjected to 1 g load tension and washed with 5 ml Krebs solution every 20 min. After 1 h equilibration, progesterone or antagonists were added in the tissue chamber separately. The antagonists were added 3 min before using progesterone (50 μmol·L~(-1)). RESULTS: Progesterone decreased the resting tension of fundus and body longitudinal muscle (LM) (P<0.05). It inhibited the mean contractile amplitude of body and antrum LM and circular muscle (CM), and the motility index of pyloric CM (P<0.05). The inhibition of progesterone on the mean contractile amplitude could be partially blocked by phentolamine in LM of the stomach body (the mean contractile amplitude of body LM decreased from -7.5+-5.5 to -5.2+-4.5 P<0.01), and by phentolamine or indomethacin in CM of body (The inhibition of progesterone on the mean contractile amplitude of body CM decreased from -5.6+-3.0 to -3.6+-2.7 by phentolamine and from -5.6+-3.0 to -3.5+-2.5 by indomethacin, P<0.01). Hexamethonium, propranolol and L-NNA (inhibitor of NO synthetase) didn't affect the action of progesterone (P>0.05). CONCLUSION: The study suggested that progesterone can inhibit the contractile activity of isolated gastric strips in rats and the mechanism seems to be a direct one except that the action on gastric body is mediated through prostaglandin and adrenergic α receptor partly.
机译：目的：研究孕酮对大鼠离体胃条收缩活性的影响。方法：处死Wistar大鼠以去除整个胃。然后，打开胃并去除粘膜层。平行于圆形或纵向纤维，从眼底，身体，胃窦和幽门切下肌肉条。将每个肌条悬浮在含有5mL Krebs溶液的组织室内。然后，同时记录胃条在组织腔中的运动。使制剂经受1g的负载张力，并每20分钟用5ml的Krebs溶液洗涤。平衡1小时后，将黄体酮或拮抗剂分别添加到组织室中。在使用孕酮（50μmol·L〜（-1））之前3分钟添加拮抗剂。结果：孕酮降低了眼底和身体纵肌（LM）的静息张力（P <0.05）。它抑制了身体和胃窦LM和环形肌的平均收缩幅度，以及幽门CM的运动指数（P <0.05）。酚对孕激素在胃体LM中的平均收缩幅度的抑制作用可以被酚妥拉明部分阻断（身体LM的平均收缩幅度从-7.5 + -5.5降低至-5.2 + -4.5 P <0.01），而酚妥拉明或吲哚美辛对人体CM的抑制作用（苯妥拉明对孕激素对人体CM的平均收缩幅度的抑制作用从-5.6 + -3.0降低至-3.6 + -2.7，而吲哚美辛从-5.6 + -3.0降低至-3.5 + -2.5 P ＜0.01）。六甲铵，普萘洛尔和L-NNA（一氧化氮合酶抑制剂）不影响孕激素的作用（P> 0.05）。结论：该研究提示孕酮可抑制大鼠离体胃条的收缩活性，其机制似乎是直接的，但对胃体的作用部分是由前列腺素和肾上腺素α受体介导的。

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