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首页> 外文期刊>Bulletin of the National Research Centre >Supercritical Fluid Extraction of the Egyptian Rosemary (Rosmarinus officinalis) Leaves and Nigella sativa L. Seeds Volatile Oils and their Antioxidant Activities
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Supercritical Fluid Extraction of the Egyptian Rosemary (Rosmarinus officinalis) Leaves and Nigella sativa L. Seeds Volatile Oils and their Antioxidant Activities

机译：埃及迷迭香（Rosmarinus officinalis）叶和黑夜蛾种子挥发油的超临界流体提取及其抗氧化活性

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摘要

Volatile oils of rosemary (Rosmarinus officinalis) leaves and Nigella sativa L seeds were isolated by supercritical fluid extraction (SFE) under optimum conditions. The isolated volatile oils were analysed by gas chromatography-mass spectrometry (GC-MS). The main components in rosmary oil are: 1,8-cineol (54.82%), a-pinene (6.54%), caryophyllene (z) (5.48%) while the components, in Nigella are, thymo-quinone (41.05%), p-cymene (10.64%) and caryophyllene (1.89%). The estimated compounds in both oil are grouped into four main classes, namely, monoterpene hydrocarbons, light oxygenated compounds, heavy oxygenated compounds and sesquiterpene hydrocarbons. The light oxygenated compounds in both oils recorded the highest values compared with the other classes, where 1,8-cineol and thymoquinone were the main components of this group in both rosemary and Nigella volatile oils, respectively. Nigella volatile oil contains a considerable phenolic content (2.69%); mostly carvacrol (1.41%) and thymodihydro-quinone (1.15%). Also, rosemary leaves oil showed remarkable content of compounds with phenolic nucleus (borneol 6.17%, camphor 13.52% and eugenol 0.72%). Both volatile oils revealed antioxidant and radical scavenging activities on 1,1-diphyenyl-2-picryl-hydrazyl (DPPH) at various concentrations compared with the known synthetic antioxidants such as butyl hydrox-yanisol (BHA). However, the observed remarkable antioxidant activities and free radical inhibitor effect of rosemary and Nigella oils might be due to the considerable concentration of phenolic contents.
机译：在最佳条件下，通过超临界流体萃取（SFE）分离了迷迭香（Rosmarinus officinalis）叶片和黑夜蛾种子的挥发油。通过气相色谱-质谱法（GC-MS）分析分离出的挥发油。迷迭香油中的主要成分为：1,8-肉桂醇（54.82％），α-pine烯（6.54％），石竹烯（z）（5.48％），而在黑藻中的成分为胸腺醌（41.05％），对伞花烃（10.64％）和石竹烯（1.89％）。两种油中的估计化合物分为四大类，即单萜碳氢化合物，轻度氧化的化合物，重度氧化的化合物和倍半萜碳氢化合物。与其他类别相比，两种油中的轻度含氧化合物均记录了最高的值，在迷迭香和Nigella挥发油中，分别以1,8-肉桂醇和胸腺醌为该组的主要成分。 Nigella挥发油含有相当大的酚含量（2.69％）；大部分为香芹酚（1.41％）和百苯二氢醌（1.15％）。而且，迷迭香叶油显示出具有酚核的化合物的显着含量（冰片为6.17％，樟脑为13.52％，丁子香酚为0.72％）。与已知的合成抗氧化剂（例如丁基羟基-邻苯二酚（BHA））相比，这两种挥发油均对各种浓度的1,1-二噻吩基-2-吡啶-肼基（DPPH）均具有抗氧化剂和自由基清除活性。然而，观察到的迷迭香和Nigella油具有显着的抗氧化活性和自由基抑制剂作用，这可能是由于酚类成分的浓度较高。

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