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首页> 外文期刊>Bulletin of the National Research Centre >Distribution of Acidic Proteases in Gramineae. Kinetics of Protein Degradation in Wheat Triticum aestivum (cv. Giza 164) During Germination
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Distribution of Acidic Proteases in Gramineae. Kinetics of Protein Degradation in Wheat Triticum aestivum (cv. Giza 164) During Germination

机译：禾本科中酸性蛋白酶的分布。小麦小麦发芽过程中蛋白质降解动力学（吉萨164）

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摘要

Acidic protease activity was quantitatively screened in 28 species and cultivars of Gramineae family during germination. Most cultivars of cereals and some grasses as Oryza saliva and Arena fatua, not economic plants, had high acidic protease activity and could be a potentially good starting material for preparation of proteases. Kinetics of protein degradation in Triticum aestivum (cv. Giza 164) seeds during germination was examined. The results revealed that protease activity was dramatically increased from the second day of germination and reached its maximum activity on day 10. This increase in protease activity was accompanied by a decrease in soluble protein content. Gibberellic acid (GA3) caused an obvious increase in protease activity during germination that reached a 1.8 fold increase on day 10 over the untreated seeds concomitant with high decrease of the protein content. The isoenzyme pattern of T. aestivum protease during germination showed the differences in number and relative mobility of isoenzyme bands in absence and presence of GA_3. The number of isoenzymes was increased from 9 to 12 in presence of GA_3. Only 3 isoenzymes were detected in absence and presence of GA_3. The electrophoretic pattern of protein showed that the number and intensity of bands were increased specially on days 8 and 10 and number of low molecular weight bands also appeared from days 4 to 14 in presence of GA_3. The results of germination suggest that the acidic proteases play a major role in the degradation of storage protein.
机译：在发芽过程中，对禾本科科的28个物种和品种中的酸性蛋白酶活性进行了定量筛选。大多数谷物和一些草的栽培品种，例如稻谷唾液和阿雷塔纳草，而不是经济植物，都具有高酸性蛋白酶活性，并且可能是制备蛋白酶的潜在良好起始原料。检查了发芽过程中普通小麦（Giza 164）的蛋白质降解动力学。结果表明，从发芽的第二天起，蛋白酶的活性急剧增加，并在第10天达到最大活性。蛋白酶活性的增加伴随着可溶性蛋白质含量的减少。赤霉素（GA3）导致发芽过程中蛋白酶活性的明显增加，在第10天比未处理的种子增加了1.8倍，同时蛋白质含量大大降低。在没有GA_3存在的情况下，普通小麦发芽过程中的同工酶模式显示出同工酶带的数量和相对迁移率的差异。在GA_3存在下，同工酶的数量从9增加到12。在不存在和存在GA_3的情况下仅检测到3种同工酶。蛋白质的电泳图谱表明，在GA_3存在的情况下，条带的数量和强度在第8和10天特别增加，并且在第4至14天也出现了低分子量条带的数量。发芽的结果表明酸性蛋白酶在存储蛋白的降解中起主要作用。

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