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首页> 外文期刊>Bulletin de la Societe Zoologique de France >L'ADIPOGENESE DANS LA GLANDE DIGESTIVE DU MOLLUSQUE BIVALVE PECTEN MAXIMUS. Ⅱ. IMPLICATIONS MOLECULAIRES
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L'ADIPOGENESE DANS LA GLANDE DIGESTIVE DU MOLLUSQUE BIVALVE PECTEN MAXIMUS. Ⅱ. IMPLICATIONS MOLECULAIRES

机译：双壳蜂最大消化动物消化腺中的成脂作用。 Ⅱ。分子含义

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A monthly histological survey over a physiological year of the digestive cells of the scallop Pecten maximus showed that this organ stored and consumed lipid reserves according to a seasonal rhythm. For the first time in mollusc studies, specific molecular probes have been developed and used to monitor lipid storage and consumption. The results have been compared to endogenous phenomena (proliferation/apoptosis of digestive cells and gametogenesis) and exogenous ones (phytoplanktonic blooms). The molecular probes developed were : glyceraldehyde-3-phosphate dehydrogenase (GPD), implied in fatty acid acylation ; actin, a fundamental element of the cytoskeleton ; aldolase, a reversible enzyme facilitating pyruvate and dihydroxyacetone formation ; and cyclin B, an ubiquitous protein of the cell cycle, a-amylase was used to try to establish a relationship between all these cellular events and exogenous feeding inputs. A positive relationship between the gene transcription of GPD and aldolase was inversely related to the transcription of actin, confirming results obtained for mammal models. In Pecten maximus, the transcripted mRNA levels of the GPD and the aldolase fastly decreased after the spring spawning, while the transcripted mRNA of actin increased. With the phytoplanktonic blooms, transcripted mRNA levels of a-amylase increased. Those of cyclin B indicated that a proliferation of the digestive cells was initiated in winter before the transfer or release of lipids and apoptosis. These molecular tools are shown to be suitable and effective for in vivo studies of the physiology of nutrition and in particular, adipogenesis in relation to gametogenesis.
机译：每月对扇贝最大果肉消化细胞进行的组织学调查显示，该器官根据季节性节律来储存和消耗脂质储备。在软体动物研究中，第一次是开发了特定的分子探针并将其用于监测脂质的储存和消耗。将该结果与内源性现象（消化细胞的增殖/凋亡和配子发生）和外源性现象（浮游植物水华）进行了比较。开发的分子探针为：甘油3-磷酸脱氢酶（GPD），暗示脂肪酸酰化；肌动蛋白，是细胞骨架的基本元素；醛缩酶，一种促进丙酮酸和二羟基丙酮形成的可逆酶； cyclin B是细胞周期中一种普遍存在的蛋白质，α-淀粉酶被用于建立所有这些细胞事件与外源性喂养输入之间的关系。 GPD和醛缩酶的基因转录之间的正相关与肌动蛋白的转录成反比，从而证实了哺乳动物模型获得的结果。在最大果胶中，春季产卵后GPD和醛缩酶的转录mRNA水平迅速下降，而肌动蛋白的转录mRNA则增加。随着浮游植物的开花，α-淀粉酶的转录mRNA水平增加。细胞周期蛋白B的那些表明，消化细胞的增殖在冬天开始，在脂质的转移或释放以及细胞凋亡之前。这些分子工具显示出对营养生理学，特别是与配子发生有关的脂肪形成的体内研究是合适和有效的。

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