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首页> 外文期刊>Calcified Tissue International >Quantitative Evaluation of the Biocompatible and Osteogenic Properties of a Range of Biphasic Calcium Phosphate (BCP) Granules Using Primary Cultures of Human Osteoblasts and Monocytes
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Quantitative Evaluation of the Biocompatible and Osteogenic Properties of a Range of Biphasic Calcium Phosphate (BCP) Granules Using Primary Cultures of Human Osteoblasts and Monocytes

机译：使用人类成骨细胞和单核细胞的原代培养物对一系列双相磷酸钙（BCP）颗粒的生物相容性和成骨特性进行定量评估

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摘要

A range of 50% porous γ-sterilized biphasic calcium phosphate (BCP) granules, (20, 50, 80, and 100% tricalcium phosphate, TCP) were classified into two distinct size ranges, small 2–4 mm in diameter and large 4–6 mm in diameter, and their potential as bone graft extender materials was assessed in vitro using culture systems of primary-derived peripheral human blood monocytes and human osteoblasts isolated from bone. The effect of the in vitro culture conditions was evaluated prior to the introduction of the test substrates. The cellular response was assessed via quantification of viable cell adhesion to the materials, lactate dehydrogenase (LDH), the production and release of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), and prostaglandin E2 (PGE2). The higher content TCP materials, 80% and 100% TCP, had a detrimental effect on viable cell adhesion after day 1, which was not related to calcium release from the granules within the local environment. TCP granules (20% and 50%) initiated a controlled level of inflammatory response that sustained and promoted viable macrophage adhesion throughout the test period, The percentage of TCP within the BCP granules was a governing factor in determining the cellular response.
机译：一系列50％的经过γ消毒的多孔双相磷酸钙（BCP）颗粒（20％，50％，80％和100％磷酸三钙，TCP）被分为两个不同的尺寸范围，直径小2–4 mm，大4使用体外培养的原代人外周血单核细胞和从骨中分离的人成骨细胞的培养系统，对直径约为–6 mm的材料及其作为骨移植扩展材料的潜力进行了体外评估。在引入测试底物之前，先评估体外培养条件的影响。通过定量活细胞对材料的粘附，乳酸脱氢酶（LDH），白介素1β（IL-1β），肿瘤坏死因子α（TNF-α）和前列腺素E2（PGE2）的产生和释放来评估细胞反应）。较高含量的TCP材料（80％和100％TCP）对第1天后的存活细胞粘附有不利影响，这与局部环境中颗粒中钙的释放无关。 TCP颗粒（20％和50％）启动了可控制水平的炎症反应，在整个测试期间持续并促进了可行的巨噬细胞粘附。BCP颗粒中TCP的百分比是确定细胞反应的控制因素。

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