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Cilia containing 9 + 2 structures grown from immortalized cells

机译：纤毛含有永生细胞生长的9 + 2结构

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摘要

Cilia depend on their highly differentiated structure, a 9 + 2 arrangement, to remove particles from the lung and to transport reproductive cells. Immortalized cells could potentially be of great use in cilia research. Immortalization of cells with cilia structure containing the 9 + 2 arrangement might be able to generate cell lines with such cilia structure. However, whether immortalized cells can retain such a highly differentiated structure remains unclear. Here we demonstrate that (1) using E1a gene transfection, tracheal cells are immortalized; (2) interestingly, in a gel culture the immortalized cells form spherical aggregations within which a lumen is developed; and (3) surprisingly, inside the aggregation, cilia containing a 9 + 2 arrangement grow from the cell's apical pole and protrude into the lumen. These results may influence future research in many areas such as understanding the mechanisms of cilia differentiation, cilia generation in other existing cell lines, cilia disorders, generation of other highly differentiated structures besides cilia using the gel culture, immortalization of other ciliated cells with the E1a gene, development of cilia motile function, and establishment of a research model to provide uniform ciliated cells.
机译：纤毛依靠其高度分化的结构（9 + 2排列）从肺部去除颗粒并运输生殖细胞。永生化细胞可能在纤毛研究中具有重要用途。具有包含9 + 2排列的纤毛结构的细胞永生化可能能够产生具有这种纤毛结构的细胞系。然而，永生化的细胞是否可以保留这种高度分化的结构仍不清楚。在这里，我们证明（1）使用E1a基因转染，气管细胞永生化； （2）有趣的是，在凝胶培养中，永生细胞形成球形聚集体，在其中形成管腔。 （3）令人惊讶的是，在聚集体内，包含9 + 2排列的纤毛从细胞的顶极长出并伸入管腔。这些结果可能会影响许多领域的未来研究，例如了解纤毛分化的机制，在其他现有细胞系中产生纤毛，纤毛疾病，使用凝胶培养法生成纤毛以外的其他高度分化的结构，用E1a永生其他纤毛细胞。基因，纤毛运动功能的发展以及建立提供均匀纤毛细胞的研究模型的建立。

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