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The role of water channel proteins and nitric oxide signaling in rice seed germination

机译：水通道蛋白和一氧化氮信号传导在水稻种子萌发中的作用

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摘要

Previous studies have demonstrated the possible role of several aquaporins in seed germination. But systematic investigation of the role of aquaporin family members in this process is lacking. Here, the developmental regulation of plasma membrane intrinsic protein (PIP) expression throughout germination and post-germination processes in rice embryos was analyzed. The expression patterns of the PIPs suggest these aquaporins play different roles in seed germination and seedling growth. Partial silencing of the water channel genes, OsPIP1;1 and 0sPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions. Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination. Our data also revealed that the nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), promoted seed germination; furthermore, the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, inhibited germination and reduced the stimulative effects of SNP and GSNO on rice germination. Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds. These results suggest that water channels play an important role in seed germination, acting, at least partly, in response to the NO signaling pathway.
机译：先前的研究证明了几种水通道蛋白在种子萌发中的可能作用。但是，缺乏对水通道蛋白家族成员在该过程中的作用的系统研究。在这里，分析了水稻胚整个发芽和发芽后质膜内在蛋白（PIP）表达的发育调控。 PIP的表达模式表明这些水通道蛋白在种子发芽和幼苗生长中发挥不同的作用。水通道基因OsPIP1; 1和0sPIP1; 3的部分沉默可减少种子发芽，而OsPIP1; 3的过表达则在缺水条件下促进种子发芽。此外，空间表达分析表明，OsPIP1; 3在种子萌发过程中主要在胚胎中表达。我们的数据还显示，一氧化氮（NO）供体，硝普钠（SNP）和S-亚硝基谷胱甘肽（GSNO）促进了种子发芽；此外，NO清除剂2-（4-羧苯基）-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物抑制发芽并降低了SNP和GSNO对水稻发芽的刺激作用。外源NO刺激发芽种子中OsPIP1; 1，OsPIP1; 2，OsPIP1; 3和OsPIP2; 8的转录。这些结果表明水通道在种子萌发中起重要作用，至少部分地响应NO信号传导途径。

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