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首页> 外文期刊>Cell Research >Functional analysis of two Sp1/Sp3 binding sites in murine Nanog gene promoter.
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Functional analysis of two Sp1/Sp3 binding sites in murine Nanog gene promoter.

机译：鼠Nanog基因启动子中两个Sp1 / Sp3结合位点的功能分析。

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摘要

Nanog gene plays a key role in maintaining pluripotency of ES cells and early embryonic cells. A 5' flank sequence of the Nanog gene has been reported to be regulated differentially, and two regulatory elements within the Nanog promoter, namely Oct-4 and Sox-2 binding sites, have been identified to regulate the transcriptional activity of Nanog gene. In this report, we identified the role of two putative Sp1 binding sites located in the Nanog gene 5'-flanking region in regulation of murine Nanog gene transcription. Mutation studies showed that the two sites were essential for the Nanog promoter activity. Gel shift and supershift analysis showed that both sites specifically bind Sp1 and Sp3. Furthermore, overexpression of dominant-negative Sp1 or Sp3 mutants significantly inhibits Nanog promoter activity. These results suggest that the transcription factor Sp1 and Sp3 are important for Murine Nanog gene expression.Cell Research (2006) 16: 319-322. doi:10.1038/sj.cr.7310040; published online 16 March 2006.
机译：Nanog基因在维持ES细胞和早期胚胎细胞的多能性中起关键作用。据报道，Nanog基因的5'侧翼序列受到差异调节，并且已确定Nanog启动子中的两个调节元件，即Oct-4和Sox-2结合位点，可调节Nanog基因的转录活性。在此报告中，我们确定了位于Nanog基因5'侧翼区域的两个推定Sp1结合位点在调节鼠Nanog基因转录中的作用。突变研究表明，这两个位点对于Nanog启动子活性至关重要。凝胶位移和超位移分析表明，两个位点特异性结合Sp1和Sp3。此外，显性负Sp1或Sp3突变体的过表达显着抑制了Nanog启动子的活性。这些结果表明转录因子Sp1和Sp3对于鼠Nanog基因表达是重要的。Cell Research（2006）16：319-322。 doi：10.1038 / sj.cr.7310040;在线发布于2006年3月16日。

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  • 来源
    《Cell Research》 |2006年第3期|P.319-322|共4页
  • 作者

    Wu da Y; Yao Z;

  • 作者单位

    1Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Genes; Binding Sites; Analysis of substances; Flank; NOS; 基因; 结合部位;

    机译：Genes;Binding Sites;Analysis of substances;Flank;NOS;基因;结合部位;

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