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首页> 外文期刊>Cell Research >Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates
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Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates

机译：量子点免疫偶联物在正常细胞和癌细胞中的凡尔林成像

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Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their ⅰ) broad absorption spectra allowing visualization with single light source, ⅱ) exceptional photo-stability allowing long term studies and ⅲ) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.
机译：量子点是纳米粒子，最近在细胞生物学和生物医学中替代有机荧光探针出现，具有一些预测优势。这些特性包括：ⅰ）宽吸收光谱，可使用单个光源进行可视化；ⅱ）出色的光稳定性，可进行长期研究；ⅲ）狭窄且对称的发射光谱，受其大小和材料组成控制。这些独特的特性允许使用单个激发光源同时激发不同大小的量子点，同时分辨和可视化为不同的颜色。目前只有少数研究在细胞成像中测试了量子点。我们在这里描述了在正常细胞和癌细胞的mortalin成像中量子点的使用。在正常细胞和癌细胞中均带有质点的Mortalin染色模式模仿了用有机荧光探针获得的结果，并且相当稳定。

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