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首页> 外文期刊>Cell Research >A mRNA molecule encoding truncated excitatory ammo acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event
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A mRNA molecule encoding truncated excitatory ammo acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event

机译：从独立的启动子转录编码截短的兴奋性氨基酸载体1（EAAC1）蛋白（EAAC2）的mRNA分子

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摘要

Glutamate transporter EAAC1 removes excitatory neurotransmitter in central nervous system, and also absorbs glutamate in epithelia of intestine, kidney, liver and heart for normal cell growth. When a mouse cDNA was screened using EAAC1 cDNA fragment as probe in our lab, a transcript (GenBank U75214) encoding an EAAC1 protein with 148 residues truncated at N-terminal was cloned and named as EAAC2. Sequence analysis shows that EAAC2 has it's own start code and unique 5'UTR that is different from that of EAAC1. A mouse genomic library was screened and a positive clone including EAAC1 CDS was sequenced (GenBank AF 322393) and indicates that normal EAAC1 transcript (GenBank U73521) is transcribed from 10 exons in terms of exon Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ, Ⅷ,Ⅸ, Ⅹ, and EAAC2 transcript is consisted by exons from Ⅳ to Ⅸ as same as that of EAAC1 and with its unique exon β upstream to exon Ⅳ and exon δ downstream to Ⅸ. EAAC2 transcript has a cluster of transcriptional start sites not overlapping with the transcriptional start sites of EAAC1. These results indicate that EAAC2 is transcribed from an independent promoter but not an alternative splicing event.
机译：谷氨酸转运蛋白EAAC1去除了中枢神经系统中的兴奋性神经递质，并吸收了肠，肾，肝和心脏上皮中的谷氨酸，从而使细胞正常生长。当在我们的实验室中使用EAAC1 cDNA片段作为探针筛选小鼠cDNA时，克隆了一个编码EAAC1蛋白的转录本（GenBank U75214），其148个残基在N端被截断，并将其命名为EAAC2。序列分析表明，EAAC2具有自己的起始代码和独特的5'UTR，与EAAC1不同。筛选了小鼠基因组文库，并对包含EAAC1 CDS的阳性克隆进行了测序（GenBank AF 322393），表明从10个外显子转录了正常的EAAC1转录本（GenBank U73521），分别来自外显子Ⅰ，Ⅱ，Ⅲ，Ⅳ，Ⅴ，Ⅵ，Ⅵ ，Ⅶ，Ⅷ，Ⅸ，Ⅹ和EAAC2转录物由Ⅳ至ex的外显子组成，与EAAC1相同，其独特的外显子β位于外显子Ⅳ的上游，外显子δ位于Ⅸ的下游。 EAAC2转录物具有与EAAC1的转录起始位点不重叠的转录起始位点簇。这些结果表明，EAAC2是从一个独立的启动子转录而来的，而不是一个替代的剪接事件。

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