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首页> 外文期刊>Cell and Tissue Research >Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro
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Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro

机译：小鼠骨骼肌中基底层形成和不形成的成肌细胞的起源和层次与体外黏附能力和Pax7表达的关系

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As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD+ myogenic cells located inside the regenerative muscle fiber BL were laminin− but interstitial MyoD+ cells were laminin+. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin+ myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7−, whereas laminin− cells were adhesive (Ad) with Pax7+. Moreover, non-Ad/laminin+ and Ad/laminin− myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7+/laminin− and non-Ad/Pax7−/laminin+ myogenic cells were also examined using skeletal muscle interstitium-derived CD34+/45− (Sk-34) and CD34−/45− (Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (<1%) Pax7+ cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Adon-Ad capacity in repetitive culture. As expected, both Ad/Pax7+/laminin− and non-Ad/Pax7−/laminin+ myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7+/laminin− cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7−/laminin+ myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7+/ laminin− myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells.
机译：作为区分骨骼肌细胞群的一种新方法，在体内和体外纤维束培养物中的小鼠代偿性plant肌中检查了肌层细胞的基底层（BL）形成。位于再生肌纤维BL内的MyoD + 肌细胞是层粘连蛋白-，而间质MyoD + 细胞是层粘连蛋白 + 。电子显微镜也证实了这是结构性BL的形成。在纤维束培养物中观察到类似趋势，包括卫星细胞和间质性成肌细胞，层粘连蛋白 + 肌成纤维细胞主要表现出Pax7 -的非黏附（non-Ad）行为，而层粘连蛋白-细胞与Pax7 + 具有粘附性（Ad）。此外，还观察到非Ad / laminin + 和Ad / laminin -肌管，前者显示自发性收缩，而后者则没有。 Ad / Pax7 + / laminin -和非Ad / Pax7 - / laminin + 的起源和层次还使用骨骼肌间质衍生的CD34 + / 45 -（Sk-34）和CD34 - / 45 检查成肌细胞− （Sk-DN）多能干细胞，由未定型的成肌细胞和新鲜分离的Sk-DN细胞中的少数（<1％）Pax7 + 细胞组成。在重复培养中，两种细胞类型通过Ad / non-Ad能力分开。符合预期，Ad / Pax7 + / laminin -和非Ad / Pax7 - / laminin + 生肌细胞始终出现在Ad和非Ad细胞培养物中。但是，在非Ad细胞培养物中反复检测到Ad / Pax7 + / laminin -细胞，而没有发生相反的现象。这表明Sk-34和Sk-DN干细胞中存在非Ad / Pax7 - / laminin + 肌源细胞，它们能够产生Ad / Pax7 + /层粘连蛋白-的成肌细胞在成肌过程中作为原代成肌细胞，并位于后代细胞的上游。

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