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首页> 外文期刊>Cell and Tissue Research >Three-dimensional studies of Prop1-expressing cells in the rat pituitary primordium of Rathke's pouch
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Three-dimensional studies of Prop1-expressing cells in the rat pituitary primordium of Rathke's pouch

机译：Rathke囊袋大鼠垂体原基中Prop1表达细胞的三维研究

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Pituitary embryonic development progresses daily toward terminal differentiation exhibiting quantitative and qualitative alterations regulated by signal molecules and transcription factors expressed under temporospatial control. In this study, we analyzed the heterogeneity of the cells in the pituitary primordium of embryonic day (E) 13.5. The three-dimensional structure of the Rathke’s pouch was built up from measurements taken from multiple DAPI-stained sections and cell populations positive to stem/progenitor marker SOX2 and pituitary-specific transcription factor PROP1 were analyzed. The pituitary primordium (Rathke’s pouch) of E13.5 showed a flattened discoid shape of about 500 μm in diameter and 200 μm depth in a dorsoventral axis and consisted in about 5,800 cells. Immunohistochemistry revealed that 0.3% of the cells in Rathke’s pouch were SOX2-negative in the lateral region, whereas all cells at E12.5 were SOX2-positive. On E13.5, the shape and size of their nuclei showed a location-specific divergence: ellipsoid morphology in the median region and round morphology in the lateral region. Moreover, on E14.5, adrenocorticotropic-hormone-positive cells (the first hormone-producing cells appearing in the pituitary) contained round nuclei. These data suggest that differentiation to pituitary-hormone-producing cells from SOX2-negative cells starts in the lateral region between E12.5 and E13.5 and that the onset of differentiation is preceded by a change in nuclear shape.
机译：垂体胚胎发育每天朝着终末分化发展，表现出由颞骨控制下表达的信号分子和转录因子调节的定量和定性变化。在这项研究中，我们分析了胚胎天（E）13.5垂体原基中细胞的异质性。 Rathke囊的三维结构是通过对多个DAPI染色切片进行的测量得到的，并对干/祖细胞标记SOX2和垂体特异性转录因子PROP1呈阳性的细胞群体进行了分析。 E13.5的垂体原基（Rathke囊）呈扁平的盘状形状，直径约500μm，在腹腹轴上约200μm深度，由约5,800个细胞组成。免疫组织化学分析显示，Rathke囊袋中0.3％的细胞在外侧区域为SOX2阴性，而E12.5处的所有细胞均为SOX2阳性。在E13.5上，其核的形状和大小显示出特定于位置的差异：中位区域为椭圆形，而外侧区域为圆形。此外，在E14.5上，促肾上腺皮质激素-阳性细胞（在垂体中出现的第一个激素产生细胞）含有圆核。这些数据表明，从SOX2阴性细胞向垂体激素产生细胞的分化始于E12.5和E13.5之间的侧向区域，并且在分化开始之前先发生核形状变化。

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