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首页> 外文期刊>Cell and Tissue Research >Three-dimensional studies of Prop1-expressing cells in the rat pituitary just before birth
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Three-dimensional studies of Prop1-expressing cells in the rat pituitary just before birth

机译：出生前大鼠垂体中Prop1表达细胞的三维研究

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Recently, we demonstrated that differentiation was underway as early as embryonic day (E) 13.5 in the lateral region of the rat pituitary primordium. In this study, we analyze the heterogeneous property of cells in the pituitary at E21.5 (just before birth) leading to its biological function with the differentiation and expansion of tissue. The three-dimensional structure of the pituitary at E21.5 was built up from measurements taken from many DAPI-stained sections and cell populations positive to the stem/progenitor marker SOX2, pituitary-specific transcription factor PROP1 and paired-related homeodomain transcription factor PRX. At E21.5, the pituitary, composed of anterior and intermediate lobes, showed a flattened chestnut shape with dimensions of about 500 μm (dorsoventral axis) by 2500 μm (left-right axis) by 850 μm (rostrocaudal axis) and consisted in approximately 113,500, 16,000 and 14,800 cells in the anterior, intermediate and posterior lobes, respectively. Five cell types were observed expressing Sox2, Prop1 and Prx; these were heterogeneously distributed in the mediolateral and dorsoventral axes. In the anterior lobe, the marginal cell layer (MCL) was mostly occupied by stem/progenitor cells positive for SOX2, with the co-expression of Prop1 and/or Prx, whereas more SOX2-single-positive cells than those for PROP1 and PRX were scattered in the parenchyma. PRX-positive cells of mesenchymal origin invaded the parenchyma, together with PECAM- and NESTIN-positive cells, indicating the advance of vasculogenesis. Thus, marked developmental progress occurs regarding the transition of stem/progenitor cells in the MCL and regarding vasculogenesis in the parenchyma during the prenatal pituitary growth wave.
机译：最近，我们证明早在大鼠垂体原基的外侧区域的胚胎天（E）13.5正在进行分化。在这项研究中，我们分析了垂体细胞在E21.5（出生前）的异质性，导致其生物学功能随组织的分化和扩张而变化。从许多DAPI染色切片和对干/祖细胞标记SOX2，垂体特异性转录因子PROP1和成对相关的同源域转录因子PRX呈阳性的细胞群体中获得的测量结果建立了垂体在E21.5处的三维结构。在E21.5处，垂体由前叶和中间叶组成，呈扁平的栗子形状，尺寸约500μm（腹腹轴）×2500μm（左右轴）×850μm（左尾轴）。前，中和后叶分别有113,500、16,000和14,800个细胞。观察到五种表达Sox2，Prop1和Prx的细胞。这些分布在前外侧和后腹轴上。在前叶，边缘细胞层（MCL）大部分被SOX2阳性的干细胞/祖细胞占据，并与Prop1和/或Prx共同表达，而SOX2单阳性细胞比PROP1和PRX多散落在薄壁组织中。间充质来源的PRX阳性细胞与PECAM和NESTIN阳性细胞一起侵入实质，表明血管生成的进展。因此，在产前垂体生长波期间，有关MCL中干细胞/祖细胞的转移以及实质中的血管生成，发生了显着的发育进展。

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