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首页> 外文期刊>Cell and Tissue Research >Caffeine and nicotine decrease acetylcholine receptor clustering in C2C12 myotube culture
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Caffeine and nicotine decrease acetylcholine receptor clustering in C2C12 myotube culture

机译：咖啡因和尼古丁减少C2C12肌管培养物中乙酰胆碱受体的聚集

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As motor neurons approach skeletal muscle during development, agrin is released and induces acetylcholine receptor (AChR) clustering. Our laboratory investigates the effect of environmental agents on skeletal muscle development by using C2C12 cell culture. For the current project, we investigated both short-term and long-term exposure to caffeine, nicotine, or both, at physiologically relevant concentrations. Short-term exposure was limited to the last 48 h of myotube formation, whereas a long-term exposure of 2 weeks allowed for several generations of myoblast proliferation followed by myotube formation. Both agrin-induced and spontaneous AChR clustering frequencies were assessed. For agrin-induced AChR clustering, agrin was added for the last 16 h of myotube formation. Caffeine, nicotine, or both significantly decreased agrin-induced AChR clustering during short-term and long-term exposure. Furthermore, caffeine, nicotine, or both significantly decreased spontaneous AChR clustering during long-term, but not short-term exposure. Surprisingly, caffeine and nicotine in combination did not decrease AChR clustering beyond the effect of either treatment alone. We conclude that physiologically relevant concentrations of caffeine or nicotine decrease AChR clustering. Moreover, we predict that fetuses exposed to caffeine or nicotine may be less likely to form appropriate neuromuscular synapses.
机译：当运动神经元在发育过程中接近骨骼肌时，凝集素被释放并诱导乙酰胆碱受体（AChR）聚集。我们的实验室通过使用C2C12细胞培养研究了环境因素对骨骼肌发育的影响。对于当前的项目，我们研究了在生理相关浓度下短期和长期接触咖啡因，尼古丁或两者的情况。短期接触仅限于肌管形成的最后48小时，而2周的长期接触则允许几代成肌细胞增殖，然后形成肌管。评估了凝集素诱导的和自发的AChR聚类频率。对于凝集素诱导的AChR聚集，在肌管形成的最后16小时内添加凝集素。在短期和长期接触过程中，咖啡因，尼古丁或两者均显着降低了凝集素诱导的AChR聚集。此外，咖啡因，尼古丁或两者在长期（而非短期）暴露过程中可显着降低自发性AChR聚集。出人意料的是，咖啡因和尼古丁合用不会降低AChR簇集，而超出了任何一种单独治疗的效果。我们得出结论，咖啡因或尼古丁的生理相关浓度降低了AChR聚集。此外，我们预测暴露于咖啡因或尼古丁的胎儿可能不太可能形成适当的神经肌肉突触。

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