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Apoptosis is not the cause of seasonal testicular involution in roe deer

机译：凋亡不是ro季节性睾丸退化的原因

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摘要

Apoptosis is involved in the regulation of spermatogenesis. The involution of testes in seasonal breeders might be expected to involve enhanced apoptotic cell elimination. We have compared seasonally changing testicular apoptosis in roe deer with that in non-seasonally breeding cattle. Apoptotic cells were detected as TUNEL-positive cells by both flow-cytometric analysis and in situ localisation of fragmented DNA in tissue sections. Apoptosis-induced DNA fragments were also assessed by enzyme-linked immunosorbent assay (ELISA) in homogenised testicular parenchyma. As expected, the testis mass and the percentage of haploid cells in roe deer showed a seasonal pattern with a significant maximum during the rut (August), whereas no annual variation of these parameters was found in bulls. All three methods for determining apoptosis showed similar findings. Roe deer exhibited significant seasonal fluctuation of total apoptotic activity (ELISA, apoptotic cells per tubule cross section) with a maximum during the breeding season. However, the seasonal differences in the number of apoptotic cells corresponded to the variable total numbers of spermatogonia and spermatocytes per tubule cross section. Thus, the percentages of TUNEL-positive cells related to the combined number of both germ cell types showed no seasonal variance, as confirmed by percentages of apoptotic cells analysed flow-cytometrically. The maximum level of apoptosis during the rut in roe deer was similar to the values obtained during the invariably high spermatogenic activity in cattle. These results suggest that, in roe deer, apoptosis is not the cause of the seasonal involution of testes.
机译：凋亡参与精子发生的调节。预计季节性繁殖者的睾丸退化涉及增强的凋亡细胞消除。我们已经比较了季节性变化的ro的睾丸凋亡与非季节性繁殖的牛的睾丸凋亡。通过流式细胞术分析和组织切片中片段化DNA的原位定位，将凋亡细胞检测为TUNEL阳性细胞。还通过酶联免疫吸附测定（ELISA）在均质的睾丸实质中评估了细胞凋亡诱导的DNA片段。不出所料，ro的睾丸质量和单倍体细胞百分比显示出季节性模式，在车辙期间（8月）有明显的最大值，而在公牛中未发现这些参数的年度变化。三种确定凋亡的方法都显示出相似的发现。 er表现出明显的总凋亡活性的季节性波动（ELISA，每个小管横截面的凋亡细胞），在繁殖季节达到最大值。然而，凋亡细胞数量的季节性差异对应于每个肾小管横截面的精原细胞和精细胞总数的变化。因此，通过流式细胞仪分析的凋亡细胞的百分比证实，与两种生殖细胞类型的总数相关的TUNEL阳性细胞的百分比没有季节性变化。 ro车辙期间最大的细胞凋亡水平与牛不变高的生精活性期间获得的值相似。这些结果表明，在ro中，细胞凋亡不是睾丸季节性退化的原因。

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