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首页> 外文期刊>Chemosphere >Variables To Be Considered When Assessing The Photocatalytic Destruction Of Bacterial Pathogens
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Variables To Be Considered When Assessing The Photocatalytic Destruction Of Bacterial Pathogens

机译：评估细菌病原体的光催化破坏时应考虑的变量

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The current study sought to assess the importance of three common variables on the outcome of TiO_2 photocatalysis experiments with bacteria. Factors considered were (a) ability of test species to withstand osmotic pressure, (b) incubation period of agar plates used for colony counts following photocatalysis and (c) chemical nature of suspension medium used for bacteria and TiO_2. Staphylococcus aureus, Escherichia coli, Salmonella ser. Typhimurium and Pseudomonas aeruginosa were found to vary greatly in their ability to withstand osmotic pressure, raising the possibility that osmotic lysis may be contributing to loss of viability in some photocatalytic disinfection studies, Agar plate incubation time was also found to influence results, as bacteria treated with UV light only grew more slowly than those treated with a combination of UV and TiO_2. The chemical nature of the suspension medium used was found to have a particularly pronounced effect upon results. Greatest antibacterial activity was detected when aqueous sodium chloride solution was utilised, with ～1 × 10~6CFU mL~(-1) S. aureus being completely killed after 60 min. Moderate activity was observed when distilled water was employed with bacteria being killed after 2 h and 30 min, and no antibacterial activity at all was detected when aqueous tryptone solution was used. Interestingly, the antibacterial activity of UV light on its own appeared to be very much reduced in experiments where aqueous sodium chloride was employed instead of distilled water.
机译：当前的研究试图评估三种常见变量对细菌进行的TiO_2光催化实验结果的重要性。考虑的因素包括：（a）测试物种承受渗透压的能力；（b）光催化后用于菌落计数的琼脂平板的孵育时间；以及（c）用于细菌和TiO_2的悬浮介质的化学性质。金黄色葡萄球菌，大肠杆菌，沙门氏菌。在某些光催化消毒研究中，发现鼠伤寒杆菌和铜绿假单胞菌承受渗透压的能力差异很大，增加了渗透裂解可能导致丧失活力的可能性，还发现琼脂平板的孵育时间会影响结果，因为处理了细菌紫外线的生长仅比紫外线和TiO_2组合的生长慢。发现所用悬浮介质的化学性质对结果有特别显着的影响。当使用氯化钠水溶液时，具有最大的抗菌活性，〜1×10〜6CFU mL〜（-1）金黄色葡萄球菌在60分钟后被完全杀死。当使用蒸馏水并在2小时30分钟后杀死细菌时，观察到中等活性，而当使用胰蛋白aqueous水溶液时，根本没有检测到抗菌活性。有趣的是，在使用氯化钠水溶液代替蒸馏水的实验中，紫外线本身的抗菌活性似乎大大降低。

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