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首页> 外文期刊>Chemosphere >A previously uncultured, paper mill Propionibacterium is able to degrade O-aryl alkyl ethers and various aromatic hydrocarbons
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A previously uncultured, paper mill Propionibacterium is able to degrade O-aryl alkyl ethers and various aromatic hydrocarbons

机译：以前未经培养的造纸丙酸杆菌能够降解O-芳基烷基醚和各种芳烃

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摘要

A previously uncultured Propionibacterium was isolated from a highly diluted sample (10~(-6) mL) of activated sludge of paper mill effluent. The isolate MOB600 was able to grow on anisole, phenetole, benzene, toluene, phenol, styrene and biphenyl, although it used only limited carbon sources in the minimal media. The partial DNA sequence of 16S ribosomal RNA gene was 93% identical to Luteococcus peritoni CCUG38120 as the closest neighborhood in the family Propionibacteriaceae. Strain MOB600 produced 2-methoxyphenol and 2-ethoxyphenol seemingly in an unproductive pathway from the degradation of anisole and phenetole, respectively. It had a substrate preference to favor 3-alkoxyphenols over 2-alkoxy-phenols. Formation of 3-hydroxylated O-aryl alkyl ether was substantially proved by the nearly 1:1 bio-transformation of substrate-analogous 1,2-methylenedioxybenzene to 3,4-methylenedioxyphenol (sesamol) showing end-product inhibition. The strain converted 2-/3-methoxypheno!s to 3-methoxycat-echol. The extradiol ring fission of 3-methoxycatechol appeared to take place in the production of a yellow-colored 2-hydroxymuconate derivative, thereby being able to release methanol spontaneously. High specificity polymerase chain reaction screening for bacterial dioxygenases revealed that the genomic DNA encoded at least three ring-hydroxylating dioxygenase large subunits. Being consistent with substrate availability for this strain, the obtained sequences were closely related to large subunits of an iso-propylbenzene 2,3-dioxygenase, a benzene 1,2-dioxygenase, a biphenyl 2,3-dioxygenase, a benzoate 1,2-dioxygenase and a putative dioxygenase in Rhodococcus strains. Our results demonstrate that strain MOB600 may play a major role in the degradation of lignin-like O-aryl alkyl ethers and various aromatic hydrocarbon pollutants in activated sludge of paper mill effluent.
机译：从造纸厂废水的活性污泥中高度稀释的样品（10〜（-6）mL）中分离出先前未培养的丙酸杆菌。分离物MOB600能够在苯甲醚，苯酚，苯，甲苯，苯酚，苯乙烯和联苯上生长，尽管它在基本培养基中仅使用了有限的碳源。 16S核糖体RNA基因的部分DNA序列与腹膜肺炎球菌CCUG38120（Propionibacteriaceae家族中最近的邻域）的93％相同。菌株MOB600似乎是通过苯甲醚和苯酚的降解分别以非生产性途径产生2-甲氧基苯酚和2-乙氧基苯酚。它具有底物偏爱3-烷氧基苯酚而不是2-烷氧基苯酚。通过与底物类似的1,2-亚甲基二氧基苯几乎1：1生物转化为3,4-亚甲基二氧基苯酚（芝麻酚），可以证明3-羟基化的O-芳基烷基醚的形成，显示出最终产物的抑制作用。该菌株将2- / 3-甲氧基苯酚转化为3-甲氧基cat-echol。在产生黄色的2-羟基粘康酸酯衍生物的过程中似乎发生了3-甲氧基邻苯二酚的二醇外环裂变，从而能够自发地释放甲醇。对细菌双加氧酶的高特异性聚合酶链反应筛选显示，基因组DNA编码至少三个环-羟化双加氧酶大亚基。与该菌株的底物可用性一致，获得的序列与异丙基苯2,3-双加氧酶，苯1,2-双加氧酶，联苯2,3-双加氧酶，苯甲酸酯1,2的大亚基密切相关。 -双加氧酶和推定的双加氧酶。我们的结果表明，菌株MOB600可能在造纸废水的活性污泥中的木质素样O-芳基烷基醚和各种芳香烃污染物的降解中起主要作用。

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  • 来源
    《Chemosphere》 |2009年第10期|1287-1293|共7页
  • 作者

    Nithyananda Thorenoor; Yong-Hak Kim; Cheolju Lee; Myeong-Hee Yu; Karl-Heinrich Engesser;

  • 作者单位

    Life Sciences Division, Korea Institute of Science and Technology, Seongbuk, Seoul 136-791, Republic of Korea;

    Functional Proteomics Center, Korea Institute of Science and Technology, Seongbuk, Seoul 136-791, Republic of Korea;

    Life Sciences Division, Korea Institute of Science and Technology, Seongbuk, Seoul 136-791, Republic of Korea;

    Functional Proteomics Center, Korea Institute of Science and Technology, Seongbuk, Seoul 136-791, Republic of Korea;

    Institut fuer Siedlungswasserbau, Wasserguete-und Abfallwirtschaft, Universitat Stuttgart, Abteilung Biologische Abluftreinigung (ALR), Bandtale 2, D-70569 Stuttgart (Buesnau). Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    aromatic hydrocarbons; O-aryl alkyl ether degradation; propionibacterium; uncultured bacterium;

    机译：芳香烃邻芳基烷基醚降解;丙酸杆菌未培养细菌;

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