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首页> 外文期刊>Chemosphere >Proteomic analysis of chromate-induced modifications in Pseudokirchneriella subcapitata
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Proteomic analysis of chromate-induced modifications in Pseudokirchneriella subcapitata

机译：蛋白质组学分析人参假单胞菌中铬酸盐引起的修饰

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摘要

In this work, we have analyzed the changes in the protein expression profile elicited by chromium (Cr) exposure in the freshwater green alga Pseudokirchneriella subcapitata, a well known bio-indicator of water pollution. We tested two experimental conditions, namely 0.2 and 1 ppm of potassium dichromate; this concentration range includes the environmentally-relevant concentrations. Results show that neither concentration of potassium dichromate tested inhibited algal growth. However, the proteomic approach allowed the identification of relevant modifications in protein expression. In fact, among 800 protein spots detected by two-dimensional electrophoresis, 16 Cr-regulated proteins, including predicted and novel ones, were identified using tandem mass spectromic protein analysis.rnThe results demonstrate a Cr-specific action in altering several photosynthetic proteins, such as ribu-lose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), RuBisCO activase, Light Harvesting Chla/b protein complex, and stress related Chla/b binding proteinl. Although Cr toxicity with respect to photosynthesis has been already documented, here we have identified, for the first time, the target proteins of this toxicity. Cr also induced a modulation of some proteins involved in the metabolism of the amino acids glutamine, arginine and methionine. These data are supported by changes in cellular poly-amine (PA) accumulation. Present findings provide new insight into the molecular mechanisms underlying Cr toxicity in P. subcapitata.
机译：在这项工作中，我们分析了在众所周知的水污染生物指标淡水绿藻次生伪藻中，铬（Cr）暴露引起的蛋白质表达谱的变化。我们测试了两个实验条件，即0.2和1 ppm的重铬酸钾。该浓度范围包括与环境有关的浓度。结果表明，所测试的重铬酸钾浓度均不能抑制藻类的生长。但是，蛋白质组学方法可以鉴定蛋白质表达中的相关修饰。实际上，通过二维电泳检测到的800个蛋白质斑点中，使用串联质谱分析法鉴定出16种Cr调控的蛋白质，包括预测的和新颖的蛋白质.rn结果表明Cr特异性改变了几种光合蛋白质，例如如核糖-丢失1,5,5-二磷酸羧化酶/加氧酶（RuBisCO），RuBisCO活化酶，Light Harvesting Chla / b蛋白复合物和与压力相关的Chla / b结合蛋白。尽管已经记录了Cr对光合作用的毒性，但在此我们首次鉴定了这种毒性的靶蛋白。铬还诱导了一些与氨基酸谷氨酰胺，精氨酸和蛋氨酸代谢有关的蛋白质的调节。这些数据得到细胞多胺（PA）积累量变化的支持。目前的发现提供了新的见解，在潜在的P.subcapitata Cr毒性分子机制。

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  • 来源
    《Chemosphere》 |2009年第10期|1372-1379|共8页
  • 作者

    Candida Vannini; Milena Marsoni; Guido Domingo; Fabiana Antognoni; Stefania Biondi; Marcella Bracale;

  • 作者单位

    Dipartimento Ambiente, Salute, Sicurezza, Universita degli Studi dell'lnsubria, via C.B. Vico 46, 21100 Varese, Italy;

    Dipartimento Ambiente, Salute, Sicurezza, Universita degli Studi dell'lnsubria, via C.B. Vico 46, 21100 Varese, Italy;

    Dipartimento Ambiente, Salute, Sicurezza, Universita degli Studi dell'lnsubria, via C.B. Vico 46, 21100 Varese, Italy;

    Dipartimento di Biologia evoluzionistica sperimentale, Universita degli Studi di Bologna, via Imerio 42, 40126 Bologna, Italy;

    Dipartimento di Biologia evoluzionistica sperimentale, Universita degli Studi di Bologna, via Imerio 42, 40126 Bologna, Italy;

    Dipartimento Ambiente, Salute, Sicurezza, Universita degli Studi dell'lnsubria, via C.B. Vico 46, 21100 Varese, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    chromium; heavy metal; pseudokirchneriella subcapitata; 2-DE; mass spectrometry; polyamines;

    机译：铬;重金属;假单胞菌2-DE;质谱;多胺;

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