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首页> 外文期刊>Chinese science bulletin >Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells
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Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells

机译：透射电子显微镜和原子力显微镜表征细菌细胞上镍的沉积

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Recently bacterial cells have become attractive biological templates for the fabrication of metal nano-structures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells (gram-negative bacteria of Escherichia coli) were fabricated via electroless chemical plating. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallization. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacterial surface after metallization.
机译：近来，细菌细胞由于其固有的小尺寸，各种标准的几何形状和丰富的来源，已经成为用于制造金属纳米结构或纳米材料的有吸引力的生物模板。本文通过化学镀制备了镍包被的细菌细胞（大肠杆菌革兰氏阴性细菌）。原子力显微镜（AFM）和透射电子显微镜（TEM）表征结果显示，细菌细胞在沉积镍前后有明显的形态差异。具有光滑表面的裸细胞在云母表面表现出横向扩展作用。金属化后，这些细菌细胞的表面粗糙度发生了很大变化。在活化和随后的金属化之后，观察到大量的镍纳米颗粒均匀分布在细菌表面。此外，超薄截面分析结果验证了金属化后细菌表面镍薄涂层的存在和均匀性。

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