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首页> 外文期刊>Chinese science bulletin >Interfamily pregnancy and expression of CD57, CD68 in deciduas between golden hamster and mouse
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Interfamily pregnancy and expression of CD57, CD68 in deciduas between golden hamster and mouse

机译：仓鼠和小鼠之间蜕皮中的家族间妊娠和CD57，CD68的表达

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摘要

Pregnancy between different species is one of the key steps to interspecific somatic cell cloning. Although interspecific clone embryos have been constructed, they could not develop to birth after being transferred to recipients. In order to clarify the mechanism of this phenomenon, interfamily pregnancy between golden hamste (Mesocricetus auratus) and mouse (Mm musculus) was studied. Co-culture results indicated that the adhesion ratios of golden hamster blastocysts on mouse uterine epithelia monolayer 12, 24, 48 and 72 h after co-culture were all significantly lower than those of mouse blastocysts. The outgrowth ratios of golden hamster blastocysts on mouse uterine epithelia monolayer 48, 72 h after co-culture were both significantly lower than those of mouse blastocysts (P < 0.01). Golden hamster's blastula could be implanted and develop to D 11 of pregnancy after being transferred to mouse uterus (the 7th day after embryo transfer). Compared to the transfer of mouse embryo to mouse uterus, the successful ratio of interfamily embryo transfer was lower and the bulk of fetus was smaller than that of intraspecific fetus. Compared to intraspecific pregnancy of mouse, the remote decidual tissue of interfamily pregnancy on D8 is looser. At the same time, expressions of CD57 and CD 68 in remote deciduas were both higher than those in the secondary deciduas in both intraspecific and interfamily pregnancy. However, expressions of the two molecules in interfamily pregnancy were lower than those in intraspecific pregnancy. These results showed that interfamily pregnancy could be established between golden hamster and mouse. But the development of fetus in interfamily pregnancy was slower than that in intraspecific pregnancy. The expression difference of CD57 and CD68 indicates the difference of immunoreaction between interfamily and intraspecific pregnancy, which may be one of the reasons leading to interfamily pregnancy termination.
机译：不同物种之间的怀孕是种间体细胞克隆的关键步骤之一。尽管已构建了种间克隆胚胎，但将它们转移到受体后无法发育到出生。为了阐明这种现象的机理，研究了金Hamste（Mesocricetus auratus）和小鼠（Mm musculus）之间的家族间妊娠。共培养结果表明，共培养后12 h，24 h，48 h和72 h小鼠仓鼠上皮细胞金黄仓鼠囊胚的黏附率均明显低于小鼠囊胚。共培养72 h后，小鼠子宫上皮单层上金黄仓鼠胚泡的生长率均显着低于小鼠胚泡（P <0.01）。金黄仓鼠的囊泡可以被植入并在转移到小鼠子宫后（胚胎移植后的第7天）发育到妊娠的D 11。与小鼠胚胎向子宫的转移相比，家族间胚胎转移的成功率要低，并且胎儿的体积要比种内胎儿的要小。与小鼠的种内妊娠相比，D8上家族间妊娠的远端蜕膜组织较松散。同时，在种内和家族间妊娠中，远端蜕膜中CD57和CD 68的表达均高于继发性蜕膜中的表达。然而，这两种分子在家族间妊娠中的表达低于种内妊娠中的那些。这些结果表明，在金仓鼠和小鼠之间可以建立家族间的怀孕。但是，家庭间妊娠的胎儿发育要比种内妊娠的胎儿发育慢。 CD57和CD68的表达差异表明家族间和种内妊娠之间免疫反应的差异，这可能是导致家族间终止妊娠的原因之一。

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