Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process

MENG Xiangxian; WANG Kemin; TAN Weihong; LI Jun; TANG Zhiwen; GUO Qiuping; HUANG Shasheng; LI Du

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Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process

机译：基于分子信标的核酶探针实时监测酶促裂解过程

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Ribozyme probe based on molecular beacon (MBR) for monitoring enzymatic cleavage process in real time is designed and studied. The approach relies on ri-bozyme Substrates modified at the two arms, with a fluores-cent moiety attached to the end of one arm and a non-fluorescent quenching moiety attached to the end of the other arm. MBR is employed to directly convert the cleavage information into fluorescence Signal in real time. Compared with traditional approach, this method provides a no-radiolabeling, sensitive and effective way to research on the ribozyme activity, enzymatic dynamic process and ri-bozyme function during gene therapy. The activity of the ribozyme against hepatitis C virus RNA (HCV-RNA) is studied based on this assay.

机译：设计并研究了基于分子信标（MBR）的核酶探针，用于实时监测酶切过程。该方法依赖于在两个臂上修饰的核糖酶底物，其中一个荧光部分连接到一个臂的末端，一个非荧光猝灭部分连接到另一个臂的末端。 MBR用于直接将裂解信息实时转换为荧光信号。与传统方法相比，该方法为研究基因治疗过程中的核酶活性，酶动力学过程和核酶功能提供了一种无放射性标记，灵敏有效的方法。基于该试验研究了核酶对丙型肝炎病毒RNA（HCV-RNA）的活性。

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来源
《Chinese science bulletin》 |2003年第23期|p.2581-2584|共4页
作者
MENG Xiangxian; WANG Kemin; TAN Weihong; LI Jun; TANG Zhiwen; GUO Qiuping; HUANG Shasheng; LI Du;
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作者单位

State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;

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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
ribozyme probe; molecular beacon; cleavage; real time; monitoring;

机译：核酶探针;分子信标;裂解;实时;监测;

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1. 2-Aminopurine as a real-time probe of enzymatic cleavage and inhibition of hammerhead ribozymes. [J] . Kirk SR, Luedtke NW, Tor Y Bioorganic and medicinal chemistry . 2001,第9期

机译：2-氨基嘌呤作为酶促裂解和抑制锤头状核酶的实时探针。
2. Ultrasensitive monitoring of ribozyme cleavage product using molecular-beacon-ligation system [J] . MENG XiangXian, TANG ZhiWen, WANG KeMin, Chinese science bulletin . 2007,第5期

机译：使用分子信标连接系统超灵敏地监测核酶裂解产物
3. Ultrasensitive monitoring of ribozyme cleavage product using molecular-beacon-ligation system [J] . MENG XiangXian, TANG ZhiWen, WANG KeMin, Chinese science bulletin . 2007,第5期

机译：使用分子信标连接系统超灵敏地监测核酶裂解产物
4. Development of a high throughput detection system for HIV-1 using real-time NASBA based on molecular beacons [C] . R. van Beuningeif, S. A. Marras, F. R. Kramer, Conference on Genomics and Proteomics Technologies Jan 22-23, 2001, San Jose, USA . 2001

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7. 2-Aminopurine as a real-time probe of enzymatic cleavage and inhibition of hammerhead ribozymes [O] . Sarah R. Kirk, Nathan W. Luedtke, Yitzhak Tor 2001

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Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process

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