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首页> 外文期刊>Chinese science bulletin >Ultrasensitive monitoring of ribozyme cleavage product using molecular-beacon-ligation system
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Ultrasensitive monitoring of ribozyme cleavage product using molecular-beacon-ligation system

机译：使用分子信标连接系统超灵敏地监测核酶裂解产物

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摘要

This paper reports a new approach to detect ribozyme cleavage product based on the molecular-beacon-ligation system. The molecular beacon, designed in such a way that one-half of its loop is complementary to ribozyme cleavage product, is used to monitor ligation process of RNA/DNA complex in a homogeneous solution and to convert directly cleavage product information into fluorescence signal. The method need not label ribozyme and ribozyme substrate, which is fast, simple and ultrasensitive for detection of cleavage product. Detection limit of the assay is 0.05 nmol/L. The cleavage product of hammerhead ribozyme against hepatitis C virus RNA (HCV-RNA) was detected perfectly based on this assay. Owing to its ultrasensitivity, excellent specificity, convenience and fidelity, this method might hold out great promise in ribozyme reaction and ribozyme gene therapy.
机译：本文报道了一种基于分子-信标-连接系统检测核酶裂解产物的新方法。分子信标的设计使其环的一半与核酶裂解产物互补，用于监测RNA / DNA复合物在均匀溶液中的连接过程，并将裂解产物的信息直接转化为荧光信号。该方法不需要标记核酶和核酶底物，该方法快速，简单，超灵敏，可用于检测裂解产物。该分析的检出限为0.05 nmol / L。基于该试验，可以完美地检测出锤头状核酶对丙型肝炎病毒RNA（HCV-RNA）的裂解产物。由于其超高的敏感性，优异的特异性，方便性和保真性，该方法在核酶反应和核酶基因治疗中具有广阔的前景。

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