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MALDI-TOF-MS Directly Combined with TLC: A Review of the Current State

机译：MALDI-TOF-MS直接与TLC结合：当前状态的回顾

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摘要

Thin-layer chromatography (TLC) is a widely used, fast and inexpensive method for separating complex mixtures. Unfortunately, the quality of achievable separation represents only one side. An additional problem is the unambiguous assignment of the obtained spots to defined compounds. Clear identification of spots is often not possible by common staining methods and comparison with a known reference compound. Therefore, further analytical techniques are mostly required for further structural elucidation. Mass spectrometry (MS) is a suitable method due to its high sensitivity. In particular, matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS is a modern soft-ionization technique that may be easily combined with TLC. This review summarizes the so far available knowledge about direct TLC–MALDI combination and gives an overview about different molecule classes that have already been successfully analyzed by this approach. This review critically summarizes the capabilities and limitations of the direct MALDI–TLC combination and highlights in particular the problems related to sample preparation and instrumentation.
机译：薄层色谱（TLC）是分离复杂混合物的一种广泛使用，快速且廉价的方法。不幸的是，可实现的分离质量仅代表了一侧。另一个问题是将获得的斑点明确分配给确定的化合物。通常的染色方法和与已知参考化合物的比较通常无法清楚地识别斑点。因此，为进一步阐明结构，最需要进一步的分析技术。质谱（MS）由于灵敏度高，是一种合适的方法。特别是，基质辅助激光解吸和电离飞行时间（MALDI-TOF）MS是一种现代软电离技术，可以很容易地与TLC结合使用。这篇综述总结了迄今为止有关直接TLC-MALDI组合的知识，并概述了已经通过这种方法成功分析的不同分子类别。这篇评论批判性地总结了直接MALDI-TLC组合的功能和局限性，特别强调了与样品制备和仪器相关的问题。

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