• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chromatographia >Ultrasensitive Capillary Electrophoresis of Oligoguluronates with Laser-Induced Fluorescence Detection
【24h】

Ultrasensitive Capillary Electrophoresis of Oligoguluronates with Laser-Induced Fluorescence Detection

机译：寡聚古藻酸酯的超灵敏毛细管电泳和激光诱导荧光检测。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An ultrasensitive method, capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection, has been established for analysis of oligoguluronates. The oligoguluronates were derivatized with 9-aminopyrene-1,4,6-trisulfonic acid (APTS) by reductive amination at 60°C for 3.5 h. The effect on the separation of buffer pH and buffer concentration were examined. The APTS-oligoguluronates were successfully separated within 20 min by use of acidic (pH 2.5) phosphate buffer as electrolyte and a potential of 20 kV. The method was used to monitor the oligoguluronates produced by acidic hydrolysis of homopolymeric blocks of guluronic acids.
机译：建立了一种超灵敏方法，即毛细管电泳（CE）和激光诱导荧光（LIF）检测技术，用于寡聚古龙酸酯的分析。通过在60°C还原胺化3.5 h，用9-氨基py-1,4,6-三磺酸（APTS）衍生低聚古龙酸酯。检查对缓冲液pH和缓冲液浓度分离的影响。通过使用酸性（pH 2.5）磷酸盐缓冲液作为电解质，并在20 kV的电势下，在20分钟内成功分离了APTS-寡聚古龙酸酯。该方法用于监测古洛糖醛酸均聚物嵌段的酸性水解产生的低聚古龙酸酯。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号