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首页> 外文期刊>Chromatographia >Urtica dioica Agglutinin (UDA) – Separation and Quantification of Individual Isolectins by Reversed Phase High Performance Liquid Chromatography
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Urtica dioica Agglutinin (UDA) – Separation and Quantification of Individual Isolectins by Reversed Phase High Performance Liquid Chromatography

机译：荨麻二聚体凝集素（UDA）–反相高效液相色谱法分离和定量单个分离素

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摘要

Stinging nettle (Urtica dioica and Urtica urens) roots are traditionally used as a remedy against BPH in Europe, and one of the plant's active principle discussed is UDA (a mixture of different isolectins). This paper reports on the first HPLC-method permitting the qualitative and quantitative analysis of individual isolectins in Urtica plant material. Optimum results were obtained by using a cyano column, and a mobile phase comprising of 10 mM phosphate buffer (pH 6.75) and acetonitrile. Temperature and detection wavelength were adjusted to 25 °C and 228 nm, respectively. The method was successfully validated for linearity, accuracy and precision. Several U. dioica samples were analysed, and except for one, all contained lectins (isolectins II, I, V and VI in varying composition). Individual compounds were assigned by comparison with reference material or based on data obtained from LC-MS experiments. The quantitative results showed variations in the lectin content from 0.016 to 0.401%; in three commercial products, which were additionally analyzed, no lectins were found.
机译：荨麻（Urtica dioica和Urtica urens）的根系在欧洲传统上用作抗BPH的药物，所讨论的植物有效成分之一是UDA（不同异凝集素的混合物）。本文报道了第一种HPLC方法，该方法可以对荨麻属植物材料中的单个异凝集素进行定性和定量分析。通过使用氰基柱和包含10 mM磷酸盐缓冲液（pH 6.75）和乙腈的流动相，可获得最佳结果。将温度和检测波长分别调节至25°C和228 nm。该方法已成功验证了线性，准确性和精密度。分析了几个U.dioica样品，除了一个样品外，所有样品都含有凝集素（组成不同的isolectins II，I，V和VI）。通过与参考物质进行比较或根据从LC-MS实验获得的数据来指定各个化合物。定量结果表明，凝集素含量在0.016％至0.401％之间变化。在另外进行了分析的三种商业产品中，未发现凝集素。

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