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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Urtica dioica agglutinin: Separation identification, and quantitation of individual isolectins by capillary electrophoresis and capillary electrophoresis-mass spectrometry
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Urtica dioica agglutinin: Separation identification, and quantitation of individual isolectins by capillary electrophoresis and capillary electrophoresis-mass spectrometry

机译：荨麻二聚体凝集素：毛细管电泳和毛细管电泳-质谱法的分离鉴定和单个异凝集素的定量

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摘要

With benign prostatic hyperplasia (BPH) being a major health problem in ageing men, alternative therapeutic approaches (e.g., with phytopharmaceuticals) are of great interest. Based on pharmacological evidences, one of the most promising options in that respect are the lectins found in Urtica dioica (stinging nettle) roots. In this study the qualitative and quantitative analysis of individual isolectins in U. dioica extracts is described, which is the first report on using capillary electrophoresis (CE) for the analysis of lectins in plant material at all. By utilizing a 200 mm sodium acetate buffer (pH 3.75) a baseline separation and determination of four closely related isolectins was feasible within 20 min in the aqueous plant extracts. The individual compounds were identified based on reference compounds as well as data obtained from CE-mass spectrometry (MS) experiments. After modifying the optimized CE conditions to 100 mm ammonium formate buffer with pH 3.75 and a voltage of 15 kV, the isolectins were clearly assignable in positive electrospray ionization (ESI) mode. The quantitative results obtained by CE (the total lectin content varied from 0 to 0.42% in the samples) were accurate (recovery rates of spiked samples between 92.5 and 96.2%), precise (relative standard deviation < 5%) and in good agreement to those obtained by High-performance liquid chromatography (HPLC). As for peak resolution, assignable compounds and required separation time the newly developed CE method was clearly advantageous over the determination achieved by LC.
机译：由于良性前列腺增生（BPH）是老龄男性的主要健康问题，因此替代治疗方法（例如，使用植物药物）引起了极大的兴趣。根据药理学证据，在这方面最有前途的选择之一是在荨麻叶（荨麻）根中发现的凝集素。在这项研究中，描述了U. dioica提取物中各个异凝集素的定性和定量分析，这是有关使用毛细管电泳（CE）完全分析植物材料中凝集素的第一个报告。通过使用200 mm乙酸钠缓冲液（pH 3.75），可以在20分钟内在水性植物提取物中进行基线分离和四种紧密相关的异凝集素的测定。根据参考化合物以及从CE质谱（MS）实验中获得的数据鉴定出各个化合物。在将优化的CE条件修改为pH值为3.75且电压为15 kV的100 mm甲酸铵缓冲液后，可在正电喷雾电离（ESI）模式下明确分配异凝集素。通过CE获得的定量结果（样品中总凝集素含量从0到0.42％不等）是准确的（加标样品的回收率在92.5和96.2％之间），精确的（相对标准偏差＆LT; 5％），并且一致性良好通过高效液相色谱（HPLC）获得的化合物。关于峰分离度，可分配的化合物和所需的分离时间，新开发的CE方法明显优于LC的测定方法。

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