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Differences among bread wheat genotypes for tissue nitrogen content and their relationship to grain yield and protein content

机译:面包小麦基因型组织氮含量的差异及其与籽粒产量和蛋白质含量的关系

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This study was conducted to examine genotypic differences in tissue nitrogen content of wheat and establish the extent to which they were related to variation in grain yield and protein content. Thirty-six genotypes consisting of cultivars and advanced breeding lines were grown at four rates of applied nitrogen: 0, 50, 100 and 200 kg N/ha, for 3 years. Above-ground plant matter was harvested at approximately Zadoks growth stage 31 (commencement of stem elongation), microwaved for 4 min, then oven-dried at 60¦C. Tissue nitrogen content of the hammermill-ground sample and grain protein content were determined by Near Infrared Reflectance Spectrometry. Genotypic differences in tissue nitrogen content, grain yield and protein content were established in each year. Maxima for tissue nitrogen and grain protein were obtained at the highest nitrogen rate, whereas the minima were at the zero rate. Yield responses to nitrogen application varied between years. Tissue nitrogen content was positively correlated with grain protein content in 8 of 12 within individual nitrogen rate comparisons. Over all nitrogen rates, the two variables were significantly positively correlated (ranging from r = 0.453 to r = 0.771). Tissue nitrogen content and protein content generally exhibited high heritability estimates within and over years, whereas grain yield had a high within year but a low over years heritability value. The use of tissue nitrogen content provides a possible basis for a screening test to select for yield and protein content in wheat breeding programs
机译:这项研究旨在检查小麦组织氮含量的基因型差异,并确定它们与谷物产量和蛋白质含量变化之间的相关程度。以四种施氮量(0、50、100和200 kg N / ha)生长由品种和高级育种系组成的36个基因型3年。大约在Zadoks生长第31阶段(开始茎伸长)收获地上植物,微波干燥4分钟,然后在60℃下烘干。用近红外反射光谱法测定锤磨样品的组织氮含量和谷物蛋白质含量。每年确定组织氮含量,谷物产量和蛋白质含量的基因型差异。组织氮和谷物蛋白的最大值以最高氮速率获得,而最小值以零速率获得。氮肥的产量响应随年份而变化。在单独的氮比率比较中,组织中的氮含量与12个样品中的8个的谷物蛋白含量呈正相关。在所有氮含量下,这两个变量均呈显着正相关(从r = 0.453到r = 0.771)。组织中的氮含量和蛋白质含量通常在数年内和数年内均显示出较高的遗传力估计值,而谷物产量在年内具有较高的遗传率,但多年来的遗传力值较低。组织氮含量的使用为筛选测试选择小麦育种计划的产量和蛋白质含量提供了可能的基础

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