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首页> 外文期刊>Cytology and genetics >Novel AluI-polymorphism in the fourth intron of chicken growth hormone gene
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Novel AluI-polymorphism in the fourth intron of chicken growth hormone gene

机译：鸡生长激素基因第四内含子的新型AluI多态性

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摘要

A novel AluI-polymorphism in the fourth intron of chicken growth hormone gene was shown. The cytosine to thymine transition in the restriction site for AluI was detected. Primers flanking the 460 bp fragment of the fourth intron containing a polymorphic restriction site for AluI were designed. The nucleotide sequence fragments amplified polymorphic variants were determined. Using designed primers, the genetic structure of populations of White Plymouth Rock, Poltava Clay, Rhode Island Red, and Borkovskaya Barvistaya chicken breeds were analyzed. It was found that growth hormone gene (by AluI-polymorphism in the fourth intron) was polymorphic in all experimental populations. Frequencies of alleles C and T in chicken population of White Plymouth Rock breed were 0.14 and 0.86, Rhode Island Red were 0.3 and 0.7, Poltava Clay were 0.04 and 0.96, and Borkovskaya Barvistaya were 0.08 and 0.92, respectively. The tendency to increase egg production and egg weight of chicken with C/C genotype and meat quality (live weight, carcass weight, weight of pectoral muscles) of chickens with genotype T/T of Rhode Island Red chicken breed was shown.
机译：在鸡生长激素基因的第四个内含子中显示了一种新颖的AluI多态性。在AluI的限制性位点检测到胞嘧啶向胸腺嘧啶的转变。设计了在含有AluI多态性限制性位点的第四内含子的460 bp片段两侧的引物。确定了扩增的多态性变体的核苷酸序列片段。使用设计的引物，分析了白普利茅斯岩，波尔塔瓦粘土，罗德岛红和Borkovskaya Barvistaya鸡种的种群遗传结构。发现生长激素基因（通过第四内含子的AluI多态性）在所有实验人群中都是多态的。 White Plymouth Rock品种的鸡群中等位基因C和T的频率分别为0.14和0.86，Rhode Island Red为0.3和0.7，Poltava Clay为0.04和0.96，Borkovskaya Barvistaya为0.08和0.92。显示了具有基因型T / T的罗德岛红鸡品种的C / C基因型鸡的产蛋量和蛋重增加的趋势以及肉质（活重，car体重量，胸肌重量）。

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