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首页> 外文期刊>Cytology and genetics >Obtaining of the transgenic Heliantus tuberosus L. plants, callus and 'hairy' root cultures able to express the recombinant human interferon alpha-2b gene
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Obtaining of the transgenic Heliantus tuberosus L. plants, callus and 'hairy' root cultures able to express the recombinant human interferon alpha-2b gene

机译：获得能够表达重组人干扰素α-2b基因的转基因Heliantus tuberosus L.植物，愈伤组织和“毛状”根培养物

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This work is the first to our knowledge to describe the successful attempt of Agrobacterium rhizogenes-mediated transformation of topinambour in order to obtain the transgenic H. tuberosus plants, callus and "hairy" root cultures. The plasmid vectors contained the sequence of interferon gene fused with Nicotiana plumbaginifolia L. calreticulin apoplast targeting signal driven by 35S CaMV promoter or root-specific Mll promoter. Nearly 75% obtained isolated Ri-root lines and callus cultures were proved (by PCR analysis) to contain HuINF alpha-2b transgene. We also managed to obtain H. tuberosus transgenic plants through somatic embryogenesis on the transgenic "hairy" root culture. The obtained transgenic H. tuberosus cultures exhibited highlevel antiviral activity that ranged from 2000 to 54500 IU/g FW that makes this crop considered a promising source of recombinant interferon alpha 2b protein.
机译：这项工作是我们所知的第一个描述发根农杆菌介导的topinambour转化成功的方法，以获得转基因马铃薯，愈伤组织和“毛状”根培养物的成功尝试。质粒载体包含与由35S CaMV启动子或根特异性MII启动子驱动的Nicotiana plumbaginifolia L.钙网蛋白质外体靶向信号融合的干扰素基因的序列。 （通过PCR分析）证实将近75％获得的分离的Ri-根系和愈伤组织培养物包含HuINF alpha-2b转基因。我们还设法通过在转基因“多毛”根培养上的体细胞胚发生获得了结核分枝杆菌转基因植物。获得的转基因马铃薯培养物表现出高水平的抗病毒活性，范围从2000到54500 IU / g FW，这使该作物被认为是重组干扰素α2b蛋白的有希望的来源。

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