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首页> 外文期刊>Cytology and genetics >Plant Regeneration from Hairy Roots and Calluses of Periwinkle Vinca minor L. and Foxglove Purple Digitalis purpurea L.
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Plant Regeneration from Hairy Roots and Calluses of Periwinkle Vinca minor L. and Foxglove Purple Digitalis purpurea L.

机译:从长春长春蔓长春花和毛地黄紫色洋地黄紫的长毛根和老茧再生植物。

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摘要

Plants regenerated from hairy root culture and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with varying efficiency. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. The addition of 1 mg/L BA, 0.1 mg/L NAA, and 5% sucrose to B5 medium increases regenerative capacity of periwinkle up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology, and short internodes.
机译:已经获得了从毛状根培养物和毛地黄紫色和长春花的愈伤组织再生的植物。发现毛根培养物中的器官发生在无激素的培养基上自发发生,但效率不同。从根系培养直接形成芽的频率在洋地黄中高达60%,在长春花中高达3.7%。向B5培养基中添加1 mg / L BA,0.1 mg / L NAA和5%蔗糖可使长春花的再生能力提高至19.1%。再生植物表现出通常在Ri转基因植物中常见的形态特征。它们包括根系的生长和斜生,芽形成增加,叶片形态变化和节间短。

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