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首页> 外文期刊>Cytology and genetics >A Parentage Study of Closely Related Ukrainian Wine Grape Varieties using Microsatellite Markers
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A Parentage Study of Closely Related Ukrainian Wine Grape Varieties using Microsatellite Markers

机译：利用微卫星标记对乌克兰酒葡萄品种进行亲缘关系研究

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摘要

Fourbred grapevine varieties released for commercial cultivation in Ukraine, namely 'Antey Magarachs-kii', 'Rubinovyi Magaracha', 'Granatovyi Magaracha' and 'Rubin Golodrigi', and their putative parental forms were genotyped using six microsatellite loci. Genotypes were compared with breeding records to verify genetic relationships among varieties. Results of the analysis confirmed four of six parent-offspring relationships. Results of the analysis allow to assume that genotype 'Seyve Villard 20347' is the direct parent of 'Antey Magarachskii' instead of its grandparent. The first-studied accession believed to be that of'Granatovyi Magaracha' was identified as impurity. In order to verify the parentage of 'Granatovyi Magaracha', rest accessions of that variety and its putative parent 'Antey Magarachskii' were additionally genotyped at 13 nuclear loci and at three chloroplast loci. The parent-offspring relationship was confirmed, as all 'Granatovyi Magaracha' accessions had a common allele with the parent variety 'Antey Magarachskii' at each locus and the same chlorotype A. Different 'Granatovyi Magaracha' accessions could have been obtained via vegetative propagation of two seedlings which arose from one crossing.
机译：在乌克兰发布用于商业种植的四品种葡萄品种，即“ Antey Magarachs-kii”，“ Rubinovyi Magaracha”，“ Granatovyi Magaracha”和“ Rubin Golodrigi”，并使用六个微卫星基因座对它们的推定亲本形式进行了基因分型。将基因型与育种记录进行比较，以验证品种之间的遗传关系。分析结果证实了六个亲子关系中的四个。分析结果允许假设基因型“ Seyve Villard 20347”是“ Antey Magarachskii”的直接亲本，而不是其祖父母。被认为是杂质的第一个研究对象是“ Granatovyi Magaracha”。为了验证“ Granatovyi Magaracha”的亲本，另外在13个核基因座和3个叶绿体基因座上对该基因的其余种及其推定的亲本“ Antey Magarachskii”进行了基因分型。确认了亲子关系，因为所有'Granatovyi Magaracha'品种在每个基因座均具有一个共同的等位基因，且与亲本品种'Antey Magarachskii'相同，并且具有相同的A型叶绿素。一次杂交产生了两棵幼苗。

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  • 来源
    《Cytology and genetics》 |2010年第2期|95-102|共8页
  • 作者

    S. Goryslavets; V. Risovanna; R. Bacilieri; J.-F. Hausman; M. Heuertz;

  • 作者单位

    National Institute for Vine and Wine ' Magarach', Ampelography, Breeding and Genetics of Grapevine, Yalta, Ukraine;

    National Institute for Vine and Wine ' Magarach', Ampelography, Breeding and Genetics of Grapevine, Yalta, Ukraine;

    Institut National de la Recherche Agronomique, Centre INRA de Montpellier, 2 Place Viala, 34000 Montpellier, France;

    Centre de Recherche Public-Gabriel Lippmann, Environment and Agro-Biotechnology, rue du Brill 41, L-4422 Belvaux, Luxembourg;

    Centre de Recherche Public-Gabriel Lippmann, Environment and Agro-Biotechnology, rue du Brill 41, L-4422 Belvaux, Luxembourg Universite Libre de Bruxelles, Evolutionary Biology and Ecology cp 160/12, av. F.D. Roosevelt 50, B-1050 Brussels, Belgium Centre of Forest Research CIFOR-INIA, Forest Systems and Resources, ctra. De la Corna km 7.5, E- 28040 Madrid, Spain;

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