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首页> 外文期刊>Analytica chimica acta >Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers
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Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers

机译：使用核和叶绿体微卫星标记检测和鉴定葡萄汁和葡萄酒中的葡萄品种

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摘要

Characteristics such as production yield,alcoholic level,acidity and anthocyanin levels vary among grape varieties.Since these characteristics are highly correlated with the final wine quality,it is important to be able to detect and correctly identify the grape varieties (cultivars) present in musts and wines.This is particularly relevant in controlling the quality and authenticity of monovarietal wines.In this study,molecular methods based on residual DNA analysis of Vitis vinifera L.cultivars were applied to musts and wines produced from five different cultivars,namely Touriga Franca,Fernao Pires,Tinta Barroca,Tinto Cao and Marselan.Initially,three DNA extraction methods were compared and optimised for the isolation of DNA from must and wine and then six nuclear and two chloroplast microsatellite markers were used to identify single-varietal and blends of two varieties of musts prepared in laboratory and single-varietal wines produced in microvinifications.Preliminary results on multivarietal musts indicated a possible relationship between the proportion of each variety in the mixture and the signal intensity of the alleles obtained in an automatic sequencer,suggesting that it could be possible to quantify the presence of each variety in the mixture.A method for DNA extraction from wine was developed and shown to be adequate for microsatellite amplification.Results have shown for the first time that markers targeting short DNA fragments at chloroplast genomes are useful tools to detect grapevine DNA in wines.This suggests that it may be possible to apply these techniques in controlling wine quality and origin certification,as well as in detecting wine falsification.
机译：葡萄品种之间的产量，酒精度，酸度和花青素含量等特性各不相同。由于这些特性与最终葡萄酒的品质高度相关，因此能够检测并正确识别葡萄汁中存在的葡萄品种（品种）非常重要。在控制单品种葡萄酒的质量和真实性方面，这一点尤为重要。在这项研究中，将基于葡萄品种葡萄残留DNA分析的分子方法应用于从五个不同品种（即图里加·弗朗卡（Touriga Franca），首先，比较和优化了三种DNA提取方法以从葡萄汁和酒中分离DNA，然后使用六个核和两个叶绿体微卫星标记物鉴定单品种和两种的混合物实验室酿造的各种葡萄汁和微酒酿造的单品种葡萄酒。mul的初步结果杂种必须表明混合物中每个品种的比例与自动测序仪中获得的等位基因信号强度之间的可能关系，这表明可以量化混合物中每个品种的存在。DNA提取方法结果表明，针对叶绿体基因组中短DNA片段的标记物是检测葡萄酒中葡萄DNA的有用工具，这首次表明了这种葡萄酒的微卫星扩增效果。在控制葡萄酒质量和原产地证书以及检测葡萄酒伪造中。

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