In Silico Analysis Of The Structure Of Variable Domains Of Mouse Single-chain Antibodies Specific To The Human Recombinant Interferon β1b

A. I. Flyak; M. V. Pavlova; P. V. Gilchuk

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In Silico Analysis Of The Structure Of Variable Domains Of Mouse Single-chain Antibodies Specific To The Human Recombinant Interferon β1b

机译：人重组干扰素β1b特异的小鼠单链抗体可变域结构的计算机模拟分析

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In silico analysis of the DNA encoding single-chain Fv antibodies (ScFv) specific to the human recombinant interferon β1b and α2b (rhIFN-β1b, rhIFN-α2b) has been carried out. The V-, D- and J-gene segments, the complementarity-determining (CDR) and framework (FR) regions, n-nucleotides as well as mutation rates which take place during the affinity maturation of the examined sequences have been determined. For the panel of ScFv against rhIFN-β1b isolated from an immune combinatorial cDNA library uniqueness of the CDRH3 loop by the length and amino acid composition has been shown. Multiple alignments with the nearest homologies from the NCBI databases have revealed that the sequences of ScFv obtained are new.

机译：在计算机上分析了编码对人重组干扰素β1b和α2b（rhIFN-β1b，rhIFN-α2b）特异性的单链Fv抗体（ScFv）的DNA。已经确定了V-，D-和J-基因区段，互补决定区（CDR）和构架区（FR），n-核苷酸以及在所检查序列的亲和力成熟过程中发生的突变率。对于从免疫组合cDNA文库分离的针对rhIFN-β1b的ScFv的面板，已经显示了CDRH3环的长度和氨基酸组成的独特性。与来自NCBI数据库的最近同源性的多次比对表明，获得的ScFv序列是新的。

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《Cytology and genetics》 |2009年第1期|42-47|共6页
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A. I. Flyak; M. V. Pavlova; P. V. Gilchuk;
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7. In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon β1b [O] . Flyak A.I., Pavlova M.V., Gilchuk P.V. 2009

机译：对重组人干扰素β1b特异的小鼠单链抗体可变域结构进行计算机分析

In Silico Analysis Of The Structure Of Variable Domains Of Mouse Single-chain Antibodies Specific To The Human Recombinant Interferon β1b

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