Agrobacterium-Mediated Transformation of Lettuce (Lactuca saliva L.) with Genes Coding Bacterial Antigens from Mycobacterium tuberculosis

N.A. Matvieieva; M.Yu. Vasylenko; A. M. Shakhovsky; N. V. Kuchuk

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Agrobacterium-Mediated Transformation of Lettuce (Lactuca saliva L.) with Genes Coding Bacterial Antigens from Mycobacterium tuberculosis

机译：农杆菌介导的莴苣（Lactuca saliva L.）转化编码结核分枝杆菌细菌抗原的基因

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Transgenic lettuce plants (Lactuca sativa L.) with genes coding the synthesis of tuberculous antigens were obtained using the Agrobacterium-mediated transformation procedure. Cotyledonary leaves of in vitro lettuce seedlings (cvs. Eralash, Snezhinka, and Rubinovoe Kruzhevo) were transformed with plasmids containing nptⅡ. a selective neomycin phosphotransferase Ⅱ gene, and ESAT6, Ag85B (-TMD), and ESAT6:Ag85B (-TMD) target genes. A PCR analysis of the genome's DNA confirmed the presence of both selective and target genes in all plants examined. At the same time, RT-PCR analysis showed that, in the case of stable transcription of the nptll gene, both the presence and absence of transcription of the ESAT6 gene are possible.

机译：使用农杆菌介导的转化方法获得具有编码结核性抗原合成的基因的转基因生菜植物（莴苣）。用含有nptⅡ的质粒转化莴苣幼苗的子叶（品种为Eralash，Snezhinka和Rubinovoe Kruzhevo）。选择性新霉素磷酸转移酶Ⅱ基因，以及ESAT6，Ag85B（-TMD）和ESAT6：Ag85B（-TMD）靶基因。对基因组DNA的PCR分析证实，在所有检查过的植物中都存在选择性基因和靶基因。同时，RT-PCR分析表明，在nptll基因稳定转录的情况下，ESAT6基因转录的存在与否都是可能的。

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《Cytology and genetics》 |2009年第2期|94-98|共5页
作者
N.A. Matvieieva; M.Yu. Vasylenko; A. M. Shakhovsky; N. V. Kuchuk;
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Institute of Cellular Biology and Genetic Engineering, National Academy of Sciences of Ukraine, ul. Akademika Zabolotnogo 148, Kyiv, 03143, Ukraine;

Institute of Cellular Biology and Genetic Engineering, National Academy of Sciences of Ukraine, ul. Akademika Zabolotnogo 148, Kyiv, 03143, Ukraine;

Institute of Cellular Biology and Genetic Engineering, National Academy of Sciences of Ukraine, ul. Akademika Zabolotnogo 148, Kyiv, 03143, Ukraine;

Institute of Cellular Biology and Genetic Engineering, National Academy of Sciences of Ukraine, ul. Akademika Zabolotnogo 148, Kyiv, 03143, Ukraine;

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3. Effective Agrobacterium-Mediated Transformation of Chicory (Cichorium intybus L.) with Mycobacterium Tuberculosis Antigene ESAT6 [J] . N. A. Matvieieva, M. Y. Vasylenko, A. M. Shahovsky, Cytology and genetics . 2011,第1期

机译：农杆菌介导的结核分枝杆菌抗原ESAT6对菊苣（菊苣）的有效农杆菌介导转化。
4. AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGARCANE (SACCHARUM OFFICINARUM L.) WITH BACTERIAL PHYTASE GENE [C] . DWI ANDREAS SANTOSA, ROY HENDROKO, ABDELAZIM FAROUK, International Society of Sugar Cane Technologists Congress vol.2; 20050130-0204; Guatemala City(GT) . 2005

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6. The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning sequence determination and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. [O] . J Content, A de la Cuvellerie, L De Wit, 1991

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7. Wpływ szczepów bakterii wyizolowanych z hydroponicznej uprawy sałaty (Lactuca sativa L.) na wzrost siewek sałaty, rosnących w obecnosci rożnych form pożywienia azotowego [Influence of bacterial strains isolated from hydroponic cultures of lettuce (Lactuca sativa L.) on the growth of lettuce seedlings growing in the presence of various forms of nitrogen nutrition] [O] . Z. Kobierzyńska-Gołąb 2015

机译：Wpływszczepówbakteriiwyizolowanych z hydroponicznejuprawysałaty（Lactuca sativa L.）na wzrostsiewekałaty，rosnącychwobecnoscirożnych形式pożywieniaazotowego[从莴苣（Lactuca sativa L.）水培培养物中分离的细菌菌株对莴苣幼苗生长的影响存在各种形式的氮营养]

Agrobacterium-Mediated Transformation of Lettuce (Lactuca saliva L.) with Genes Coding Bacterial Antigens from Mycobacterium tuberculosis

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