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首页> 外文期刊>Desalination and water treatment >Occurrence changes of Escherichia coli (including O157:H7 serotype) in wastewater and sewage sludge by quantitation method of (EMA) real time-PCR
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Occurrence changes of Escherichia coli (including O157:H7 serotype) in wastewater and sewage sludge by quantitation method of (EMA) real time-PCR

机译：（EMA）实时荧光定量PCR法测定废水和污泥中大肠杆菌（包括O157：H7血清型）的发生变化

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摘要

There are hundreds of Escherichia coli serotypes and among them enterohaemorrhagic strain E. coli O157:H7 causing gastrointestinal disorders such as: bloody diarrhea, cramping and abdominal pain and the infectious "hemolytic uremic syndrome". Therefore, it's important to develop some rapid and reliable methods of detecting this pathogen in wastewater and sewage sludge. This will allow one to determine the potential risk of infection for humans and animals as far as wastewater and sewage management is concerned. E. coli non- and -O157:H7 gene copies were detected in primary influents and final effluents in winter from municipal wastewater treatment plant. The ethidium monoazide bromide (EMA) application revealed false-positive detection of this bacteria in final effluents. In spring and summer, E. coli gene was not found either in wastewater or in sludge. In autumn, E. coli genes were found in primary influents (20,000 copies of gene/100 mL) and final effluents (2,511 copies of gene/100 mL). High amounts of E. coli O157:H7 gene were detected in both kinds of sludge: waste activated sludge (3,890,451 copies of gene/100 mL) and final sewage sludge (1,819,700 copies of gene/100 mL). We detected the large amount of "free DNA" that is derived from dead cells, which can give false-positive results (overestimation). The use of EMA will make it possible to avoid this inconvenience and allow for effective and appropriate selection of EMA concentration, however, it needs further analysis.
机译：有数百种大肠杆菌血清型，其中肠出血性大肠杆菌O157：H7引起胃肠道疾病，例如：血性腹泻，绞痛和腹痛以及传染性“溶血性尿毒症综合征”。因此，重要的是开发一些快速可靠的方法来检测废水和污泥中的这种病原体。就废水和污水管理而言，这将使人们能够确定对人类和动物的潜在感染风险。在冬季市政污水处理厂的主要进水和最终出水中检测到了大肠杆菌的非和-O157：H7基因拷贝。溴化单叠氮化乙锭（EMA）的应用揭示了最终废水中该细菌的假阳性检测。在春季和夏季，在废水或污泥中均未发现大肠杆菌基因。秋季，在主要进水（20,000个基因/ 100 mL）和最终出水（2,511个基因/ 100 mL）中发现了大肠杆菌基因。在两种污泥中均检测到大量的大肠杆菌O157：H7基因：废活性污泥（3,890,451个基因/ 100 mL）和最终污水污泥（1,819,700个基因/ 100 mL）。我们检测到了大量来自死亡细胞的“游离DNA”，这些DNA可能会给出假阳性结果（高估）。 EMA的使用将有可能避免这种不便，并允许有效和适当地选择EMA浓度，但是，还需要进一步分析。

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  • 来源
    《Desalination and water treatment》 |2014年第21期|3965-3972|共8页
  • 作者

    K.L. Fijalkowski; M.J. Kacprzak; A. Rorat;

  • 作者单位

    Institute of Environmental Engineering, Czestochowa University of Technology, Brzeznicka 60a, Czestochowa 42-200, Poland;

    Institute of Environmental Engineering, Czestochowa University of Technology, Brzeznicka 60a, Czestochowa 42-200, Poland;

    Institute of Environmental Engineering, Czestochowa University of Technology, Brzeznicka 60a, Czestochowa 42-200, Poland;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Wastewater; Sewage sludge; EMA; real time-PCR; Escherichia coli;

    机译：废水;污水污泥;EMA;实时PCR大肠杆菌;

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