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首页> 外文期刊>Biological Procedures Online >Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells
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Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

机译：通过电穿孔进行人脂肪干细胞和间充质干细胞竞争性DNA转染制剂

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BackgroundAdipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use. ResultsWe developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells. ConclusionsOur findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation.
机译：背景脂肪干细胞在基于细胞的治疗中具有强大的潜力，但是目前依赖未知缓冲液的核转染技术阻止了它们的使用。结果我们使用以前开发的三步法开发了一种用于人脂肪干细胞的最佳核转染制剂。设计该方法的目的是确定能够满足或超过既定的商业缓冲液（Amaxa）的核转染的最佳配方，尤其是对于鼠类脂肪干细胞。通过使用相同的缓冲液，我们确定相同的制剂在人间充质干细胞中产生最佳的转染效率。结论我们的发现表明，适当配制可提高人干细胞的转染效率。

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